Functional Enhancer Screening in Single Cells

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Functional Enhancer Screening in Single Cells Joris van Arensbergen, Bas van Steensel Molecular Cell Volume 66, Issue 2, Pages 167-168 (April 2017) DOI: 10.1016/j.molcel.2017.03.018 Copyright © 2017 Elsevier Inc. Terms and Conditions

Figure 1 Schematic Representation of the Mosaic-Seq Strategy (A) In a population of cells, different sgRNAs repress different enhancers by the recruitment of dCas9-KRAB, in some instances leading to a reduction of expression of a target gene. The presence of different sgRNAs is traced by a barcode that is present in the same construct as the sgRNA. (B) By combining the data from hundreds of single-cell RNA-seq (scRNA-seq) profiles for each sgRNA, the effect of each enhancer on each gene can be assessed. Molecular Cell 2017 66, 167-168DOI: (10.1016/j.molcel.2017.03.018) Copyright © 2017 Elsevier Inc. Terms and Conditions