Loss of tight junctions on Rac1 activation in mature cysts.

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Presentation transcript:

Loss of tight junctions on Rac1 activation in mature cysts. Loss of tight junctions on Rac1 activation in mature cysts. Localization of green fluorescent protein (GFP)‐occludin during mitosis in a mature cyst. (A) Time‐lapse confocal images of an Madin–Darby canine kidney (MDCK) cyst expressing GFP‐occludin. (B,C) MDCK cysts expressing FK506‐binding protein (FKBP)‐Tiam1 (B) and FKBP‐β2‐chimaerin (C) together with GFP‐occludin were imaged using a confocal microscope at 0, 20 and 40 h without or with 100 nM dimerizer. Arrowheads indicate retained and newly formed tight junctions. Lu indicates a cyst lumen. Scale bar, 10 μm. (D) Cysts expressing FKBP‐Tiam1 were cultured for 11 days and further cultured in the absence and presence of 100 nM dimerizer, fixed and stained with anti‐ZO‐1 antibody (magenta) and Hoechst33258 (green). Scale bar, 10 μm. (E) A model of Rac1 activity regulation for morphological change during cystogenesis. Shunsuke Yagi et al. EMBO Rep. 2012;13:237-243 © as stated in the article, figure or figure legend