Ultra-rapid vitrification of mouse oocytes in low cryoprotectant concentrations Ho-Joon Lee, Heidi Elmoazzen, Diane Wright, John Biggers, Bo R Rueda,

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Presentation transcript:

Ultra-rapid vitrification of mouse oocytes in low cryoprotectant concentrations Ho-Joon Lee, Heidi Elmoazzen, Diane Wright, John Biggers, Bo R Rueda, Yun Seok Heo, Mehmet Toner, Thomas L Toth Reproductive BioMedicine Online Volume 20, Issue 2, Pages 201-208 (February 2010) DOI: 10.1016/j.rbmo.2009.11.012 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Quartz capillary (QC) vitrification device and oocyte loading method. (a) The QC device; (b) schematic illustration of the QC loaded with oocytes; and (c) photomicrograph of oocytes loaded into QC and loaded oocytes (arrowheads). VS, vitrification solution. Reproductive BioMedicine Online 2010 20, 201-208DOI: (10.1016/j.rbmo.2009.11.012) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Oocyte survival rate after vitrification in slush nitrogen in the two different vitrification solutions, 0.5mol/l trehalose+1.5mol/l 1,2-propanediol (PrOH) in HEPES-buffered physiological salt solution containing 20% fetal bovine serum (FHM20) or 0.5mol/l trehalose+2.0mol/l PrOH in FHM20, immediately and 2h after warming. Reproductive BioMedicine Online 2010 20, 201-208DOI: (10.1016/j.rbmo.2009.11.012) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions