Ebolavirus’s Foibles Cell Seiya Yamayoshi, Yoshihiro Kawaoka  Cell  Volume 169, Issue 5, Pages 773-775 (May 2017) DOI: 10.1016/j.cell.2017.05.008 Copyright © 2017 Elsevier Inc. Terms and Conditions

Figure 1 Cross-Reactive mAbs and Their Epitopes on Ebolavirus GP (A) Epitopes on the structure of ebolavirus GP. The ebolavirus GP trimer (PDB: 5JQ3) is composed of three heterodimers each of GP1 (blue) and GP2 (light blue). The base, glycan cap (deep blue), head (which includes the receptor-binging site), and mucin-like domain (not shown in the 3D structure) of GP1 and the internal fusion loop, HR1, and HR2 of GP2 are mapped on the structure. The target sites of the reported cross-reactive mAbs are mapped on the GP structure. Critical contact sites or amino-acid changes found in escape mutants for ADI-15750, ADI-15968, BDBV289, BDBV43, BDBV324, and BDBV370 are shown in yellow (G271, W275, Y241, and L273). Amino-acid changes found in escape mutants for ADI-15946 (K510, orange), 6D6 (G528 and A530, pink), ADI-15878, and ADI15742 (G528, pink) are also highlighted. Critical contacts for CA45 include R64, Y517, G546, and N550 (green). ADI-16061 and BDBV223 bind to HR2. ADI-16061 is evaded by mutations in H628 and D632 (yellow; D632 position estimated). There are no known escape mutants for BDBV223. These 3D figures were provided by courtesy of Erica Ollmann Saphire (The Scripps Research Institute). (B) Monoclonal antibodies with pan-ebolavirus activities. Wec et al. (2017) obtained ADI-15742 and ADI-15878 from a human EBOV survivor. ADI-15742/15878 recognize the tip of the GP2 internal fusion loop (pink) and neutralize all five ebolaviruses: Ebola virus (EBOV), Bundibugyo virus (BDBV), Sudan virus (SUDV), Taï Forest virus (TAFV), and Reston virus (RESTV). CA45 recognizes the root of the GP2 internal fusion loop (green) and neutralizes four of the five ebolaviruses (RBOV, BDBV, SUDV, and RESTV). Cell 2017 169, 773-775DOI: (10.1016/j.cell.2017.05.008) Copyright © 2017 Elsevier Inc. Terms and Conditions