Analytical bias of cross-reactive polyclonal antibodies for environmental immunoassays of Alternaria alternata Detlef Schmechel, PhD, Brett J. Green,

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Presentation transcript:

Analytical bias of cross-reactive polyclonal antibodies for environmental immunoassays of Alternaria alternata Detlef Schmechel, PhD, Brett J. Green, PhD, Francoise M. Blachere, MSc, Erika Janotka, MUDr, Donald H. Beezhold, PhD Journal of Allergy and Clinical Immunology Volume 121, Issue 3, Pages 763-768 (March 2008) DOI: 10.1016/j.jaci.2007.09.046 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Representative inhibition curves of pAb binding to solid-phase A alternata extract by spores commonly found in indoor environments. The results represent the average of 4 replicates, and the error bars indicate the SEMs. Journal of Allergy and Clinical Immunology 2008 121, 763-768DOI: (10.1016/j.jaci.2007.09.046) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Immunoblot analysis of pAb reactivity to mycelial extracts of selected fungi. MWM, Molecular weight markers; 1, S botryosum; 2, U chartarum; 3, A alternata; 4, commercial A alternata extract, 5, A brassicicola; 6, Cladosporium cladosporioides; 7, C lunata; 8, P variotii; 9, P variotii with no pAb (negative control). Journal of Allergy and Clinical Immunology 2008 121, 763-768DOI: (10.1016/j.jaci.2007.09.046) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 High-resolution images of halogen immunostaining (green fluorescence) of spores of (A and B)A alternata, (C)E nigrum, (D)U chartarum, (E)Curvularia lunata, (F) germinated C lunata, (G)P variotii, and (H)Cladosporium herbarum using an anti–A alternata pAb. Scale bar, 10 μm. Journal of Allergy and Clinical Immunology 2008 121, 763-768DOI: (10.1016/j.jaci.2007.09.046) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions