A kinase‐dead mutant and an ECO‐associated mutant of ICK show a decreased ability to rescue shortened cilia phenotype in ICK−/− MEFs A–FKinase activity.

Slides:

Advertisements

Similar presentations

Survivin is a determining factor for mitotic localization of the chromosome passenger complex. Survivin is a determining factor for mitotic localization.

Advertisements

Protection of HIF‐1α against VHL‐mediated degradation requires nuclear translocation of HIF‐1α and a hypoxia‐dependent intranuclear signal. Protection.

Defective phenotypes of ts alp4 and alp6 mutants and the cellular localization of Alp4 and Alp6 at the MTOC. (A) Wild‐type (left, HM123, Table II), alp4‐1891.

Colocalization of the human trimethylguanosine synthase 1 and survival of motor neuron proteins. Colocalization of the human trimethylguanosine synthase.

Internalization of kinase‐dead epidermal growth factor receptor K721A

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

The N‐terminus and SH2 domain of SOCS‐1 contribute to inhibition of JAK1 and JAK2 kinase activity. The N‐terminus and SH2 domain of SOCS‐1 contribute to.

Loss of USP8 or HIF1α impairs endocytic recycling required for ciliogenesis Kinetic analysis of transferrin recycling measured by flow cytometry. Loss.

Full‐length XIAPs that retain caspase 9 inhibitory potential inhibit UV‐induced cell death. Full‐length XIAPs that retain caspase 9 inhibitory potential.

Dynamics of ookinete to oocyst transition, and effects of SRPN2 knockdown. Dynamics of ookinete to oocyst transition, and effects of SRPN2 knockdown. Mosquitoes.

Relocalisation of both MK5 and ERK3 requires a CRM1‐dependent nuclear export pathway. Relocalisation of both MK5 and ERK3 requires a CRM1‐dependent nuclear.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

Characterization and phenotypes of sima mutants.

Loss of GW body staining in Drosha‐deficient HeLa cells and transfection of synthetic short interference RNAs rescues GW bodies. Loss of GW body staining.

Nore1 enhances interaction between HIPK1 and Mdm2.

Interactions between Aiolos and Ikaros proteins are detected within the cell nucleus. Interactions between Aiolos and Ikaros proteins are detected within.

Impairment of Listeria protrusion formation by overexpression of the cytoplasmic tail of CD44. Impairment of Listeria protrusion formation by overexpression.

ERM proteins are essential for efficient cell‐to‐cell spread of Listeria. ERM proteins are essential for efficient cell‐to‐cell spread of Listeria. (A–D)

MCPH1 interacts with E2F1. MCPH1 interacts with E2F1. (A,B) HEK293 cells were left untreated (No tx) or treated with 300 ng/ml NCS for 4 h or 2 μM adriamycin.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

Anaphase chromosome separation in extracts takes place in the absence of intact spindle poles. Anaphase chromosome separation in extracts takes place in.

Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi mutant Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi.

Topisirovic et al., (2003) EMBO J,22:

FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL.

(A) Schematic representation of full‐length APC

FFAT‐containing proteins recruit the ER‐resident MOSPD2 protein to interorganelle contact sites FFAT‐containing proteins recruit the ER‐resident MOSPD2.

Volume 15, Issue 2, Pages (August 2008)

Improved design and localization of mtZFNs Schematic structure of previous mtZFN architecture. Improved design and localization of mtZFNs Schematic structure.

KIF1Bβ promotes DHX9 nuclear localization.

The mutant ZIP13 protein is degraded through a VCP‐dependent mechanism Identification of VCP/Cdc48/p97 as a ZIP13‐associating protein. The mutant ZIP13.

VAP silencing restores a normal plasma membrane cholesterol level in STARD3‐expressing cells VAP silencing restores a normal plasma membrane cholesterol.

Subcellular localization of UUKV, HRTV, and SFTSV NSs

MET inhibition promotes p21 nuclear translocation

Cbl protein levels in ago3, twin, and armi mutant GSCs

Intrinsic role of Aub in GSC self‐renewal and differentiation

Aub represses Cbl expression in the GSCs

RB regulates half‐life and stability of Pdx‐1 protein.

Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs.

Knock‐in mutation of the T1348N mutation that ablates GTP binding to LRRK2 abolishes Rab10 and biomarker phosphorylation Knock‐in mutation of the T1348N.

Cep97 and CP110 Suppress a Cilia Assembly Program

ZBTB48 is required for MTFP1 expression

Characterization of immunosorted NCAM1+ subpopulation A

WDR11 is required for ciliogenesis

LXRα enhances glucose uptake and O‐GlcNAc signaling via activation of the hexosamine biosynthetic pathway (HBP) in cultured cardiomyocytes LXRα enhances.

Activation of Drp1 by E2F5‐Drp1 complex enhances HPV‐E7/ceramide‐mediated lethal mitophagy Activation of Drp1 by E2F5‐Drp1 complex enhances HPV‐E7/ceramide‐mediated.

GSC tumor phenotype in ago3 mutant germaria

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

AS aggregates interact with SERCA in SH‐SY5Y cells

ILK knockdown decreases mTOR signaling in PKD kidneys.

PhoB also translocates to plant nuclei in leaf and crown cells in response to HK activation with exogenous cytokinin. PhoB also translocates to plant nuclei.

NLK positively regulates YAP activity in physiological settings

Fig. 7. Knockdown of Meis1 abolishes CR4. 2-GFP expression

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Fig. 8. Knockdown of Meis1 reduces the expression of Foxn4 and Lim1+2

Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and.

Fig. 3. Rnd proteins induce stronger responses in subconfluent endothelial cells.HUVECs were transfected with Rnd1, Rnd2, Rnd3 or GFP-encoding plasmids.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Volume 34, Issue 4, Pages (August 2015)

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

CENP-F staining in prometaphase and metaphase spermatocytes from B6 and Rb/+ mice. CENP-F staining in prometaphase and metaphase spermatocytes from B6.

Fig. 2. Acetylation stiffens primary cilia.

Integrin β3 is dispensable for RhoA activity and contractility.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Inhibition of γ-secretase increased hair cell differentiation by upregulating Math1. a, Expression of Math1 in inner ear stem cells was increased after.

SiRNA directed at Ngn1 inhibited differentiation of inner ear stem cells to β-III tubulin-positive cells. a, Inner ear stem cells treated with siRNA to.

S-Affs colocalize with SUMO in mammalian cells.

AMITOL added polyubiquitin chains to K481 of IRE1α

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Identification of the molecular regulators of FLP formation.

Presentation transcript:

A kinase‐dead mutant and an ECO‐associated mutant of ICK show a decreased ability to rescue shortened cilia phenotype in ICK−/− MEFs A–FKinase activity of ICK is required for ciliary formation but not for localization in the cilia. A kinase‐dead mutant and an ECO‐associated mutant of ICK show a decreased ability to rescue shortened cilia phenotype in ICK−/− MEFs A–FKinase activity of ICK is required for ciliary formation but not for localization in the cilia. (A) Schematic representation of a kinase‐dead ICK. (B–D) Constructs expressing FLAG‐tagged GFP (B), wild‐type ICK (ICK‐WT; C), or kinase‐dead ICK K33R (ICK‐KD; D) were transfected into ICK−/− MEFs. Localization of FLAG‐tagged proteins was observed using anti‐FLAG (green) and anti‐acetylated α‐tubulin (red) antibodies. (E) The number of cilia stained with an anti‐acetylated α‐tubulin antibody was counted. (F) The length of cilia stained with anti‐acetylated α‐tubulin antibody was measured. G–OThe ECO‐associated mutant of human ICK has a decreased ability to induce ciliary formation. (G) Schematic representation of the ECO‐associated mutant of human ICK. (H–J) FLAG‐tagged constructs expressing GFP (H), wild‐type human ICK (hICK‐WT; I), or ECO‐associated mutant R272Q of human ICK (hICK‐ECO; J) were transfected into NIH3T3 cells. Localization of FLAG‐tagged proteins was observed using anti‐FLAG (green) and anti‐acetylated α‐tubulin (red) antibodies. (K) The percentage of the cilia with the FLAG signal was quantified. (L–N) The FLAG‐tagged GFP (L)‐, hICK‐WT (M)‐, or hICK‐ECO (N)‐expressing plasmid was transfected into ICK−/− MEFs. (O) The number of cilia stained with anti‐acetylated α‐tubulin antibody was counted. Data information: Nuclei were stained with DAPI (blue). Scale bars, 5 μm (B–D, L–N) and 2 μm (H–J). Error bars show the SD. *P < 0.03. Arrowheads indicate ciliary tips. Taro Chaya et al. EMBO J. 2014;33:1227-1242 © as stated in the article, figure or figure legend