Volume 7, Issue 8, Pages (August 2000)

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Volume 7, Issue 8, Pages 611-621 (August 2000) At the maize/Agrobacterium interface: natural factors limiting host transformation Jin Zhang, Laural Boone, Remigiusz Kocz, Chuhan Zhang, Andrew N Binns, David G Lynn Chemistry & Biology Volume 7, Issue 8, Pages 611-621 (August 2000) DOI: 10.1016/S1074-5521(00)00007-7

Scheme 1 Structures of MDIBOA, DIMBOA and AS. Chemistry & Biology 2000 7, 611-621DOI: (10.1016/S1074-5521(00)00007-7)

Figure 1 Co-cultivation of maize seedling with A348/pSW209. Photograph of 8-day-old maize seedling after growing on the agar plate containing 10 μM AS, 80 μg/ml X-gal, and 50 μg/ml kanamycin for the final 3 days. This magnification (122.5×) shows the surface of the maize root along the left border and some of the individual colonies indicated by the arrows. After several additional days the blue color shown towards the right side of the plate spreads to the entire area. The blue background did not appear in control plates containing no bacteria. Chemistry & Biology 2000 7, 611-621DOI: (10.1016/S1074-5521(00)00007-7)

Figure 2 Co-cultivation of maize seedling with A348/pAC2. (A) Photograph of 10-day-old maize seedling after growing on the agar plate containing 100 μM AS, 0.2 μg/ml Calcofluor (1 μg/ml Calcofluor in top agar), and 50 μg/ml kanamycin for the final 5 days. (B,C) Expansion of the roots embedded in the agar with the arrow indicating the zone of depleted bacterial colonies, plated at 105 CFU/ml in the top agar. (D,E) UV illumination of the same expanded region (arrow) highlighting both the maize roots that were embedded in the agar and the bacterial colonies. Chemistry & Biology 2000 7, 611-621DOI: (10.1016/S1074-5521(00)00007-7)

Figure 3 Maize root exudate. Exudate was collected by dipping 7-day-old maize seedlings in CH2Cl2 containing 0.5% HOAc and analyzed by HPLC (Microsorb SiO2, cyclohexane/ethyl acetate (67:33 v/v)). Insert contains expansions of the minor components present in the exudate. Detection at 254 nm. Chemistry & Biology 2000 7, 611-621DOI: (10.1016/S1074-5521(00)00007-7)

Figure 4 Inhibition of vir expression by MDIBOA. β-Galactosidase induced by 1 mM AS (♢), 100 μM AS (□) and without AS (⊞) in A348-3/pAB123-22, pSW209Ω and without AS in A348-3/pMutA G665D, pSW209Ω (○). Chemistry & Biology 2000 7, 611-621DOI: (10.1016/S1074-5521(00)00007-7)

Figure 5 Mutant analysis. (A) Effect of various [MDIBOA] on virB::lacZ gene expression induced by 10 μM AS with 100 mM glucose in wild type A348 (□), JZ103 (○), and JZ104 (♢) carrying pSW209 (virB::lacZ). (B) β-Galactosidase induction by AS in wild type A348 (□), JZ103 (○), and JZ104 (♢) carrying pSW209 (virB::lacZ). Mean±standard deviation (n=3). Chemistry & Biology 2000 7, 611-621DOI: (10.1016/S1074-5521(00)00007-7)