Volume 99, Issue 6, Pages (September 2010)

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Volume 99, Issue 6, Pages 2006-2015 (September 2010) Monitoring Photosynthesis in Individual Cells of Synechocystis sp. PCC 6803 on a Picosecond Timescale S.B. Krumova, S.P. Laptenok, J.W. Borst, B. Ughy, Z. Gombos, G. Ajlani, H. van Amerongen Biophysical Journal Volume 99, Issue 6, Pages 2006-2015 (September 2010) DOI: 10.1016/j.bpj.2010.07.015 Copyright © 2010 Biophysical Society Terms and Conditions

Figure 1 DAS of BE cells, obtained after global analysis of streak-camera data. The corresponding lifetimes are given in the figure. The excitation wavelength was 400 nm. Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions

Figure 2 Global analysis results of a representative image of BE cells detected with 700-nm bandpass filter. Global analysis was performed with a three-exponential model. (A) Fluorescence intensity image. (B) Histogram of the shortest fluorescence lifetime component (25 ps). (C–E) Spatial distribution of the individual lifetime components: 25 ps (C), 287 ps (D), and 2.6 ns (E); their corresponding average amplitudes are given in brackets. Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions

Figure 3 DAS of PAL cells, obtained after global analysis of streak-camera data. The corresponding lifetimes are given in the figure. The excitation wavelength was 400 nm. Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions

Figure 4 Global analysis results of a representative image of PAL cells detected with 700-nm bandpass filter. Global analysis was performed with a four-exponential model. (A) Fluorescence intensity image. (B) Histogram of the average fluorescence lifetime, <τ>. (C–F) Spatial distribution of the individual lifetime components: 18 ps (C), 84 ps (D), 393 ps (E), and 1.73 ns (F); the corresponding average amplitudes are given in brackets. The red-outlined cell differs from most other cells with regard to lifetime. Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions

Figure 5 DAS of isolated PBSs, obtained after four-component analysis of streak-camera data. The corresponding lifetimes are given in the figure. The excitation wavelength was 590 nm. Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions

Figure 6 Global analysis results of a representative image of Synechocystis PCC 6803 WT cells detected with a 700-nm bandpass filter. Global analysis was performed with a three-exponential model. (A) Fluorescence intensity image. (B) Spatial distribution and histogram of the average lifetime. (C–E) Spatial distribution of the individual lifetime components: 71 ps (C), 246 ps (D), and 842 ps (E). Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions

Figure 7 DAS of Synechocystis WT cells, resulting from a four-component global analysis of streak-camera data, obtained upon 400-nm (A) and 590-nm (B) excitation. The corresponding lifetimes are given in the figure. Biophysical Journal 2010 99, 2006-2015DOI: (10.1016/j.bpj.2010.07.015) Copyright © 2010 Biophysical Society Terms and Conditions