Role of poly(ADP-ribosyl)ation during human spermatogenesis

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Presentation transcript:

Role of poly(ADP-ribosyl)ation during human spermatogenesis Batia Bar-Shira Maymon, D.Sc., Malka Cohen-Armon, D.Sc., Haim Yavetz, M.D., Leah Yogev, Ph.D., Beatriz Lifschitz-Mercer, M.D., Sandra E. Kleiman, Ph.D., Amnon Botchan, M.D., Ron Hauser, M.D., Gedalia Paz, Ph.D. Fertility and Sterility Volume 86, Issue 5, Pages 1402-1407 (November 2006) DOI: 10.1016/j.fertnstert.2006.03.063 Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Evaluation of testicular biopsies of azoospermic men revealing full spermatogenesis (obstructive azoospermia) (A, D), mixed atrophy (B, E), or maturation arrest at spermatocyte level (C, F). Immunohistochemical stainings for poly(ADP-ribose) polymerase 1(PARP-1) (A–C) or for poly(ADP-ribose) polymers (PAR) (D–F). In all cases, antibody–antigen interaction was visualized with the use of DAB as a substrate to generate a brown color. Sections were counterstained with Gill’s hematoxylin, which stains nuclei blue. ET = elongating spermatid; M = myoid cell; MT = mature spermatid; RT = round spermatid; SC = Sertoli cell; SPC = spermatocyte; SPG = spermatogonia. (A) Tubule demonstrating full spermatogenesis (i.e., the presence of mature spermatids). PARP-1 immunoreactivity (brown staining) is confined to germ cells nuclei and is exhibited mainly in spermatogonia and in round spermatids. (B) Tubule demonstrating full spermatogenesis; PARP-1 immunoreactivity is demonstrated in germ cell nuclei. Mature spermatids and Sertoli cells are immunonegative. (C) Tubule demonstrating maturation arrest at the spermatocyte level; PARP-1 immunoreactivity is noticed in spermatogonia and spermatocytes. (D) Full spermatogenesis; PAR immunolabeling is demonstrated predominantly in subpopulation of primary spermatocytes and in round spermatids. (E) Full spermatogenesis; PAR immunolabeling is exhibited in elongating spermatids. Sertoli cells lack the marker’s expression. (F) Maturation arrest at spermatocyte level; PAR immunolabeling is uniformly distributed in all the apparent spermatocytes. (A) original magnification, ×200; (B, C) original magnification, ×400; (D–F) original magnification, ×600. Scale bar = 30 μm. Maymon. Poly(ADP-ribosyl)ation in spermatogenesis. Fertil Steril 2006. Fertility and Sterility 2006 86, 1402-1407DOI: (10.1016/j.fertnstert.2006.03.063) Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions