Mast Cell–Fibroblast Interactions: Human Mast Cells as Source and Inducers of Fibroblast and Epithelial Growth Factors Metin Artuc, U. Muscha Steckelings,

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Mast Cell–Fibroblast Interactions: Human Mast Cells as Source and Inducers of Fibroblast and Epithelial Growth Factors Metin Artuc, U. Muscha Steckelings, Beate M. Henz Journal of Investigative Dermatology Volume 118, Issue 3, Pages 391-395 (March 2002) DOI: 10.1046/j.0022-202x.2001.01705.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Human dermal mast cells express various growth factors. Representative agarose gel showing amplification products of mRNA encoding FGF-2, FGF-7, FGF-10, and HB-EGF. No positive signals were noted for FGF-5 and HGF. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Human dermal mast cells express FGF-2 protein. FGF-2 was detected in cell supernatants as well as in homogenates by ELISA. FGF-2 could not be further augmented by stimulation with IL-4 (5 ng per ml) or SCF (50 ng per ml) or a combination of both. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 HMC-1 cells express FGF-2 protein, and FGF-2 expression is enhanced by PMA. FGF-2 was detected in cell supernatants by ELISA. A low basal secretion of FGF-2 protein could be markedly and dose-dependently enhanced by stimulation with PMA, reaching a maximum at 10 µg per ml PMA added. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Expression of FGF-7 protein by human dermal fibroblasts can be stimulated by mast cell supernatants. FGF-7 was detected in fibroblast supernatants by ELISA: (A) controls; (B) fibroblasts stimulated with PMA; (C) fibroblasts stimulated with supernatants derived from unstimulated HMC-1 cells; (D) fibroblasts stimulated with supernatants derived from HMC-1 cells stimulated with PMA (40 µg per ml). Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Tryptase stimulates FGF-2 secretion by human dermal fibroblasts. FGF-2 and FGF-7 were detected in fibroblast supernatants by ELISA. Tryptase (0.05–5 mU) stimulated FGF-2 secretion by human dermal fibroblasts with a maximum at 0.5 mU, but had no effect on FGF-7 secretion. Data are given as mean ± SEM (FGF-7, n = 3; FGF-2, n = 5). Statistical analyses were performed using Student's two-tailed t test. *p < 0.05 vs control; **p < 0.005 vs control. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Histamine stimulates FGF-7 secretion by human dermal fibroblasts. FGF-2 and FGF-7 were detected in fibroblast supernatants by ELISA. Histamine (10−4-10−9 M) dose-dependently stimulated FGF-7 secretion, with a maximum at 10−5 M, but had no effect on FGF-2 secretion. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Histamine-induced stimulation of FGF-7 secretion by human dermal fibroblasts is mediated via H1 receptors. The histamine-induced stimulation of FGF-7 secretion (10−6 M) could be completely blocked by preincubation with the H1-histamine-receptor antagonist descarboethoxyloratadine (10−6-10−8 M), whereas preincubation with the specific H2-histamine-receptor antagonist famotidin (10−6-10−8 M) had no effect. Data are given as mean ± SEM, n = 3. Journal of Investigative Dermatology 2002 118, 391-395DOI: (10.1046/j.0022-202x.2001.01705.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions