Barbara Foster, MSa, Lawrence B

Slides:

Advertisements

Similar presentations

W.Travis Cain, MDa, Greg Cable, PhDb, John J. Oppenheimer, MDc

Advertisements

Expression of FcγRIII (CD16) on human peripheral blood eosinophils increases in allergic conditions Francis Davoine, MSc, Sophie Lavigne, MSc, Jamila.

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Response to cyclosporin and low-dose methylprednisolone in aggressive systemic mastocytosis Motohiro Kurosawa, MD, PhD, PharmDa, Hiroo Amano, MD, PhDb,

Gene expression of vascular endothelial growth factor and its receptors and angiogenesis in bronchial asthma Makoto Hoshino, MDa, Yutaka Nakamura, MDa,

Tan Jinquan, MD, PhD, Henrik H. Jacobi, MD, Chen Jing, MD, Claus M

Akos Heinemann, MD, Gunter J. Sturm, MD, Martina Ofner, BSc, Eva M

Anti-IgE treatment of eosinophil-associated gastrointestinal disorders

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Expression of PD-L1 in mastocytosis

Siglec-7 is an inhibitory receptor on human mast cells and basophils

Omalizumab treatment downregulates dendritic cell FcεRI expression

Wheat ω-5 gliadin is a major allergen in children with immediate allergy to ingested wheat Kati Palosuo, MDa,b, Elina Varjonen, MDa, Outi-Maria Kekki,

Allergen binding to specific IgE in the nasal mucosa of allergic patients Alex KleinJan, BSca, Tom Godthelp, MDa, Albert van Toornenenbergen, PhDb, Wytske.

Fuad M. Baroody, MD, Se-Hoon Suh, MD, Robert M. Naclerio, MD

Human mast cells arise from a common circulating progenitor

Mass cytometry profiling the response of basophils and the complete peripheral blood compartment to peanut Leticia Tordesillas, PhD, Adeeb H. Rahman,

Omalizumab rapidly decreases nasal allergic response and FcεRI on basophils Henry Lin, MD, Kevin M Boesel, MD, Daniel T Griffith, MD, Calman Prussin,

Inflammation of small airways in asthma

Disialoganglioside GD3 is selectively expressed by developing and mature human mast cells Shunlin Ren, PhDa, Naotomo Kambe, MDa, Zhongmin Du, PhDa, Yongli.

Clinical pharmacology of H1-antihistamines in the skin

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Paul Van Durme, MDa, Jan L. Ceuppens, MD, PhDa,b, Pascal Cadot, PhDb

Anaphylactic reaction to local administration of rifamycin SV

Mast cell activation syndrome: Proposed diagnostic criteria

Expression of IFN-γ–inducible protein; monocyte chemotactic proteins 1, 3, and 4; and eotaxin in TH1- and TH2-mediated lung diseases Deborah Miotto,

News & Notes Journal of Allergy and Clinical Immunology

Christopher L. Kepley, PhDa, John C. Cambier, PhDb, Penelope A

The immunopharmacology of mild asthma

The ascomycin macrolactam pimecrolimus (Elidel, SDZ ASM 981) is a potent inhibitor of mediator release from human dermal mast cells and peripheral blood.

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Upregulation of FcϵRI on human basophils by IgE antibody is mediated by interaction of IgE with FcϵRI Donald MacGlashan, MD, PhDa, Lawrence M. Lichtenstein,

Basophils and eosinophils in allergic rhinitis

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

Expression of IL-9 receptor α chain on human germinal center B cells modulates IgE secretion Lama M. Fawaz, PhD, Ehssan Sharif-Askari, PhD, Oumnia Hajoui,

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

IgE in unselected like-sexed monozygotic and dizygotic twins at birth and at 6 to 9 years of age: High but dissimilar genetic influence on IgE levels

Costimulatory molecules in the developing human gastrointestinal tract: A pathway for fetal allergen priming Catherine A. Jones, PhD, Gillian H.S. Vance,

Assessment of clinical findings, tryptase levels, and bone marrow histopathology in the management of pediatric mastocytosis Melody C. Carter, MD, Sarah.

Molecular virology and immunology of HIV infection

Ganglioside GQ1b enhances Ig production by human PBMCs

Lisa D. Vailes, MSa, Michael T. Kinter, PhDb, L

Cutaneous inflammatory cell infiltrate in chronic idiopathic urticaria: Comparison of patients with and without anti-FcϵRI or anti-IgE autoantibodies

Classification of anti-FcϵRI and anti-IgE autoantibodies in chronic idiopathic urticaria and correlation with disease severity Ruth A. Sabroe, MDa, Edda.

News & Notes Journal of Allergy and Clinical Immunology

Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation Alex.

Case report: Streptomycin-induced anaphylactic shock during oocyte retrieval procedures for in vitro fertilization Motoyasu Iikura, MD, PhDa, Masao Yamaguchi,

Sarbjit S. Saini, MDa, Jennifer J

Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms Toby J. Williams, Catherine A. Jones,

Histamine and tryptase levels in patients with acute allergic reactions: An emergency department–based study Robert Y. Lin, MDa, Lawrence B. Schwartz,

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

The 30th anniversary of the American Board of Allergy and Immunology: Then and now Lynn Des Prez a, Charles E. Reed, MD, Lawrence B. Schwartz, MDb, John.

Intracellular IL-5 and T-lymphocyte subsets in atopic and nonatopic bronchial asthma Yutaro Shiota, MD, PhDa, Hitomi Arikita, MTa, Naokatsu Horita, MDb,

Blood eosinophils from atopic donors express messenger RNA for the α, β, and γ subunits of the high-affinity IgE receptor (FcϵRI) and intracellular, but.

Toll-like receptor 2 ligands activate human basophils for both IgE-dependent and IgE- independent secretion Anja P. Bieneman, BS, Kristin L. Chichester,

Eosinophilic gastrointestinal disease and peanut allergy are alternatively associated with IL-5+ and IL-5− TH2 responses Calman Prussin, MD, Joohee Lee,

Analysis of the lineage relationship between mast cells and basophils using the c-kit D816V mutation as a biologic signature Can N. Kocabas, MD, Akif.

Epinephrine absorption in children with a history of anaphylaxis

Perforin hyperreleasability and depletion in cytotoxic T cells from patients with exacerbated atopic dermatitis and asymptomatic rhinoconjunctivitis allergica

Seasonal intestinal inflammation in patients with birch pollen allergy

The potential clinical utility of serum α-protryptase levels

Chemical constituents of diesel exhaust particles induce IL-4 production and histamine release by human basophils Gilles Devouassoux, MDa, Andrew Saxon,

IgE+ cells in the peripheral blood of atopic, nonatopic, and bee venom–hypersensitive individuals exhibit the phenotype of highly differentiated B cells

Changes in sputum counts and airway hyperresponsiveness after budesonide: Monitoring anti-inflammatory response on the basis of surrogate markers of airway.

Penicillium nalgiovense as an occupational and contact allergen

CD11b-mediated migratory property of peripheral blood B cells

IgG4 production is confined to human IL-10–producing regulatory B cells that suppress antigen-specific immune responses Willem van de Veen, MSc, Barbara.

News & Notes Journal of Allergy and Clinical Immunology

Characterization of dendritic cells from human oral mucosa: A new Langerhans' cell type with high constitutive FcϵRI expression Jean-Pierre Allam, MDa,

Advances in mechanisms of allergic disease in 2016

Presentation transcript:

Characterization of mast-cell tryptase-expressing peripheral blood cells as basophils Barbara Foster, MSa, Lawrence B. Schwartz, MD, PhDb, Gilles Devouassoux, MDa*, Dean D. Metcalfe, MDa, Calman Prussin, MDa Journal of Allergy and Clinical Immunology Volume 109, Issue 2, Pages 287-293 (February 2002) DOI: 10.1067/mai.2002.121454 Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 1 Tryptase expression by PBLs: a representative dot plot of ungated PBLs displaying anti-IgE staining versus tryptase staining (A) and the corresponding isotype control (B) . After gating on the IgE+, lin- population (C) , histograms were examined for tryptase (bold line , D-F ) versus the isotype-matched control (shaded histogram , D-F ). Representative histograms from low (D) , intermediate (E) , and high (F) tryptase-expressing donors are shown. Tryptase staining of IgE+, lin- PBLs with (G) or without (H) permeabilization is also shown. Journal of Allergy and Clinical Immunology 2002 109, 287-293DOI: (10.1067/mai.2002.121454) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 2 Expression of mast-cell and basophil markers by tryptase-positive PBLs. Dual tryptase and Kit (A and B ) and dual tryptase and chymase staining (E and F ) of IgE+, lin- PBLs or tonsil cells, respectively, are shown. Quadrant markers were placed on the basis of corresponding isotype-matched controls. Dual 2D7 and tryptase staining of IgE+, lin- PBLs (C) , as well as of human IgE+, lin- lung mast cells (D) , along with the corresponding isotype-matched controls (G and H , respectively), are shown. Journal of Allergy and Clinical Immunology 2002 109, 287-293DOI: (10.1067/mai.2002.121454) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 3 Basophil tryptase expression as a function of disease status. After gating on IgE+, lin- basophils, tryptase expression was measured as follows: A , percentage of positive staining; B , tryptase MEAPC value. Horizontal lines denote median tryptase expression. Mast-cell tryptase MEAPC values are shown in B . Journal of Allergy and Clinical Immunology 2002 109, 287-293DOI: (10.1067/mai.2002.121454) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 4 Basophil tryptase and histamine release. Tryptase (A) and total histamine (B) release from purified basophils by means of ELISA determined as the percentage of total amount of mediator is shown. Basophil purity for donor 1 is 72.4%, for donor 2 is 80.0%, and for donor 3 is 53.6%. Journal of Allergy and Clinical Immunology 2002 109, 287-293DOI: (10.1067/mai.2002.121454) Copyright © 2002 Mosby, Inc. Terms and Conditions