Clostridia Large Gram positive

Slides:



Advertisements
Similar presentations
Lab. No.2 Sterility Testing. applied to products intended to be sterile. (Ophthalmic and Parenteral preparations) applied to products intended to be sterile.
Advertisements

Gram positive Bacilli Branching Non-Branching Actinomycetes
Dr. Amel Yacoubi.  On Gram staining: Gram positive cocci in groups (Figure n°1)  On blood agar: beta hemolysis (complete hemolysis) (Figure n°2)  On.
PHT 381 Lab# 5 Culture Media.
General Microbiology Laboratory Bacteria Oxygen Requirements.
Most Probable Number Statistical Procedure used to estimate the number of bacteria that will grow in liquid media. Gives a 95% probability that the bacterial.
F1 PRESS F1 FOR GUIDEANCE. Clostridium, Bacteriods & Antibiotics Clostridium3 Station 1: Gram stain of Clostridium perfringens4 Station 2: Spore stain.
الجامعة الإسلامية - غزة كلية العلوم قسم التحاليل الطبية الجامعة الإسلامية - غزة كلية العلوم قسم التحاليل الطبية Bacterial Oxygen Requirement By / Shadi.
Culturing bacterial Pathogens. Pathogenic Bacteria Pathogenic bacteria are bacteria that cause bacterial infections in human beings, animals and plants.
病原生物学教研室 Department of pathogenic Biology of Gannan Medical University 医学微生物学 Medical Microbiology 张文平.
II MBBS Dr Ekta Chourasia Lecturer, Microbiology
CULTURING ANAEROBES. Effects of Oxygen on Bacteria Obligate Aerobes—”strict aerobes”; oxygen required Facultative Anaerobes—both aerobic and anaerobic.
PHT 381 Lab# 5 Culture Media.
Blood Culture GROUP MEMBERS: Aimen Niaz Rafia Hafeez Adeena Shafique
Coliform Bacteria in Water
Media Preparation & Sterilization
Isolation and Culturing of Bacteria
Microbiology Microbiology lab. Skills Bacteriology 1 & Dr. Ibrahim Hassan, Microbiology PhD.
Selective and Differential media
PHT 226 Lab# 4 Culture media Streaking.
SELECTIVE, DIFFERENTIAL AND ENRICHED MEDIA
PHT 416 Lab 7. Steps Microscopic Morphology Growth Biochemical Tests Nutrient agar Blood agar Mannitol Salt Agar MacConkey’s agar.
CULTURE METHODS G.HARIPRASAD M.Sc.Med Micro M.Phil LECTURER THOOTHUKUDI GOVT. MEDICAL COLLEGE THOOTHUKUDI.
Practical 7 ST anaerobic cultivation Bacteria in relation to oxygen Anaerobic infections - sampling and transport Cultivation - establishing of anaerobic.
Clostridium - Microscopic appearance of different species. - Differentiation between species according to biochemical reactions.
Bacteria oxygen requirements
PHT 416 Lab# 4 Culture mediaCulture media Streaking Streaking.
Clostridium perfringens The agent of - Gangrene - Food poisoning Bacteria features: GPB, Capsulate, non-motile. Spores: bulging Rapidly growing (doubling.
The Gram positive spore-forming anaerobic bacilli:
PHT382 Lab. No. 4.
Aerobic Non-Spore Forming Gram-Positive Bacilli
Lab. No Gram’s Stain: Gram’s +ve Bacilli Spore forming Non spore forming AerobicAnaerobic Bacillus Clostridium Corynebacterium.
General Microbiology Laboratory Anaerobic Bacteria.
Components Preparation Inoculation
226 PHT Final Spots. Gram’s stain Results: Shape: Cocci Arrangment: irregular clusters Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism:
PHT 313 Lab (3) Gram's +ve Bacilli.
Gather all plates and tubes so we can discuss results together:
General Microbiology Laboratory 1.  Among the many enzymes that bacteria may produce are exoenzymes (those that are excreted) used to degrade large polymers.
Gram Positive Bacilli.
Lab #3. Review of Lab #2 Gram staining  record results on pg. 35 Gram positive – purple Gram negative – pink Bacillus subtilis – Escherichia coli – Klebsiella.
Bacterial Cultivation and Identification
Clostridia Large Gram positive
Endo Spore Gram positive rods
NAS Lecturer: Mr. M. Zivuku LECTURE 4 1 Objectives By the end of this unit, you should be able to ; Explain the cultivation of bacteria in terms of in.
Media!.
culturing of microorganism
Microbiology: Principles and Explorations Sixth Edition Chapter 6: Growth and Culturing of Bacteria Copyright © 2005 by John Wiley & Sons, Inc. Jacquelyn.
Facultative an-aerobic a. Facultative an-aerobic(Enterobacteriacea)
Faculty of Medicine and Health Sciences Microbiology Lab Experiment 13
Exercise 9: Aseptic Technique: Check results
Culture media.
Department of Microbiology
د. زينة فؤاد صالح.
Culture Media م.م رنا مشعل.
Bacillus , Clostridium &
Gram Positive Bacilli THE GENUS CLOSTRIDIUM.
Lab.2- Types of Chemical Methods Sterilization
SMLS LECTURE SERIES GENUS: CLOSTRIDIUM
Aerobic Non-Spore Forming Gram-Positive Bacilli
Lec. No. 9 Dr. Manahil Genus Clostridium
Anaerobic Bacteria.
Chapter 6 Microbial Growth.
Media!.
Cultivation of baceria
Media Preparation & Sterilization
Biology & biotechnology department
Practical No.14 Genus Bacillus.
Single Media & Multiple Tests
4th lecture Dr Fitua Al-Saedi
Single Media & Multiple Tests
Presentation transcript:

Clostridia Large Gram positive Straight or slightly curved rods with slightly rounded ends Anaerobic bacilli Spore bearing Spore do not germinate and growth does not normally proceed unless a suitably low redox Saprophytes Some are commensals of the animal & human gut which invade the blood and tissue when host die and initiate the decomposition of the corpse (dead body) Causes diseases such as gas gangrene, tetanus, botulism & pseudo-membranous colitis by producing toxins which attack the neurons pathways

Clostridia of medical importance

Clostridium Causing Tetanus Cl. tetani Gram positive, straight, slender rod with rounded ends All species form endospore (drumstick with a large round end) Fermentative Obligate anaerobe Motile by peritrichous flagella Grows well in cooked meat broth and produces a thin spreading film when grown on enriched blood agar Spores are highly resistant to adverse conditions Iodine (1%) in water is able to kill the spores within a few hours

Clostridium Causing Gas Gangrene

Reaction on Litmus Milk Contains Skimmed Milk (Without Fat) Litmus indicator Lactose Sugar Casein Protein Acid Base and Redox indicator

Reaction on Litmus Milk 1- Acidic Reaction Fermentation Litmus Indicator Lactose Acid Pink Color (Milk Sugar) 2- Basic Reaction Litmus Indicator Digestion Casein Alkaline amines Blue Color (Milk Protein)

Reaction on Litmus Milk Stormy Clot Formation Fermentation Acid Gas Lactose + Stormy Clot Milk Sugar Coagulation Clot Casein Milk Protein

Reaction on Litmus Milk

Nagler’s Reaction This test is done to detect the lecithinase activity The M.O is inoculated on the medium containing human serum or egg yolk (contains lecithin) The plate is incubated anaerobically at 37 C for 24 h Colonies of Cl. perfringens are surrounded by zones of turbidity due to lecithinase activity and the effect is specifically inhibited if Cl. perfringens antiserum containing  antitoxin is present on the medium

Nagler Reaction Positive Nagler Reaction Procedure of Nagler Reaction

Anaerobic Cultivation Removal of oxygen & replacing it with inert gas Anaerobic Jar It is especially plastic jar with a tightly fitted lid Hydrogen is introduced from commercially available hydrogen generators envelop 10 ml of water is added to envelop immediately before placing it in the jar Hydrogen and carbon dioxide will release and react with oxygen in the presence of catalyst to form water droplet Anaerobic indicator (Methylene blue) is placed in the jar Methylene blue is blue in oxidized state (Aerobic condition) while turns colorless in reduced state (Anaerobic condition)

Anaerobic Cultivation Culture Media containing reducing agent Thioglycollate broth It contains Sodium thioglycollate (Reducing agent) Rezazurin (redox indicator) Low percentage of Agar-Agar to increase viscosity of medium Cooked Meat Medium Meat particles (prepared from heart muscles) which contain hematin & glutathione that act as reducing agent

Growth on Fluid Thioglycolate Clostridium sporogenes Growing in Thioglycolate Medium Reducing agents in the medium absorb oxygen and allow obligate anaerobes to grow

Reaction on Cooked Meat Medium Saccharolytic reaction It causes fermentation of glycogen of muscles Production of acid and gas Meat particles remain intact e.g Cl. perfergines Proteolytic Reaction It causes digestion of meat particles Formation of black, foul smelling due to sulfur compounds

Candle Jar Anaerobic Jar

Clostridial Cellulitis

Risus Sardonicus in Tetanus Patient