Volume 41, Issue 2, Pages 259-266 (August 2004) Endothelial cell differentiation in hepatocellular adenomas: implications for histopathological diagnosis  Géraldine Gouysse, Sophie Frachon, Valérie Hervieu, Michelangelo Fiorentino, Antonia d'Errico, Jérôme Dumortier, Olivier Boillot, Christian Partensky, Walter F. Grigioni, Jean-Yves Scoazec  Journal of Hepatology  Volume 41, Issue 2, Pages 259-266 (August 2004) DOI: 10.1016/j.jhep.2004.04.014

Fig. 1 Sinusoidal pattern of endothelial cell differentiation in hepatocellular adenoma CD31 (a) is strongly expressed by the endothelial lining of an intra-tumoral artery and its adjacent vessels (long arrows); its expression along intra-tumoral vessels (large arrow) is not detectable. CD34 (b) is restricted to a few peri-arterial capillary vessels (long arrows) and is undetectable along intra-tumoral sinusoid vessels (large arrow). CD4 (c) and the receptor II for FcIgG (Cdw32) (d) are readily detectable all along intra-tumoral sinusoid vessels (arrows). Laminin-1 (e) is readily detected in the subendothelial matrix of an intra-tumoral artery (large arrow); faint deposits are also visible along intra-tumoral sinusoid vessels (thin arrows). Tenascin (f) is abundant in the subendothelial matrix of intra-tumoral sinusoid vessels (thin arrow). (Frozen tissue sections; immunoperoxidase followed by Mayer's hematoxylin, original magnifications: a, e, f, ×150; b, c, d, ×90). Journal of Hepatology 2004 41, 259-266DOI: (10.1016/j.jhep.2004.04.014)

Fig. 2 Continuous pattern of endothelial cell differentiation in hepatocellular adenoma CD31 (a) and CD34 (b) are readily expressed by the endothelial linings of both intra-tumoral arteries (large arrows) and intra-tumoral sinusoid vessels (long arrows). CD4 (c) and the receptor II for FcIgG (Cdw32) (d) are undetectable along intra-tumoral sinusoid vessels, whereas they are expressed by, respectively, scattered lymphocytes and macrophages (arrows). Laminin-1 (e) is present in the subendothelial matrix of intra-tumoral sinusoid vessels (long arrow), but is undetectable in the adjacent, non tumoral liver (NT). Tenascin (f) is undetectable along intra-tumoral sinusoid vessels (long arrows), whereas it is readily visible in the subendothelial matrix of an intra-tumoral artery (large arrow). (Frozen tissue sections; immunoperoxidase followed by Mayer's hematoxylin, original magnifications: a, b, e, ×90; c, d, f, ×150). Journal of Hepatology 2004 41, 259-266DOI: (10.1016/j.jhep.2004.04.014)

Fig. 3 Mixed pattern of endothelial cell differentiation in a case of hepatocellular adenoma At low magnification (a), this tumor is characterized by the coexistence of CD34+ vessels (long arrows) and CD34− vessels (∗) in adjacent territories. At higher magnification (b), the transition between CD34+ (long arrows) and CD34− (∗) territories is abrupt. (Formalin-fixed, paraffin-embedded tissue section; immunoperoxidase followed by Mayer's hematoxylin, original magnifications: a, ×60; b, ×130). Journal of Hepatology 2004 41, 259-266DOI: (10.1016/j.jhep.2004.04.014)