FMR1 and AKT/mTOR signalling pathways: potential functional interactions controlling folliculogenesis in human granulosa cells Julia Rehnitz, Diego D.

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Presentation transcript:

FMR1 and AKT/mTOR signalling pathways: potential functional interactions controlling folliculogenesis in human granulosa cells Julia Rehnitz, Diego D. Alcoba, Ilma S. Brum, Katrin Hinderhofer, Berthe Youness, Thomas Strowitzki, Peter H. Vogt Reproductive BioMedicine Online Volume 35, Issue 5, Pages 485-493 (November 2017) DOI: 10.1016/j.rbmo.2017.07.016 Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 COV434 cell numbers following their treatment with different concentrations of inhibitors. Untreated cells or cells treated only with dimethyl sulfoxide (DMSO) were used as controls. The first two points on the graphs represent cell numbers before the initiation of inhibitor treatment. Reproductive BioMedicine Online 2017 35, 485-493DOI: (10.1016/j.rbmo.2017.07.016) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Gene expression changes in human granulosa cells following treatment with recombinant FSH (rFSH). Changes in the expression levels of AKT (P = 0.05), mTOR (P = 0.015), S6K (P = 0.05) and FMR1 (P = 0.031), between controls and cells treated with 0.3 IU/ml of rFSH. *P ≤ 0.05, compared with the control. Normally distributed data are presented as bar diagrams (mean ± SE); all other data are presented as box-and-whisker plots (median and interquartile range). Reproductive BioMedicine Online 2017 35, 485-493DOI: (10.1016/j.rbmo.2017.07.016) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 SK6 and FMR1 gene expression profiles in human granulosa cells, following the treatment of cells with rapamycin and MK. Fold changes, after the treatment of COV434 cells with MK and rapamycin, AKT and mTORC1 inhibitors, of S6K (P = 0.003 and P = 0.016, respectively) and FMR1 (P = 0.003 and P = 0.001, respectively) gene expression levels. *P ≤ 0.05, compared with the control. Reproductive BioMedicine Online 2017 35, 485-493DOI: (10.1016/j.rbmo.2017.07.016) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 FMRP expression. FMRP expression level was after the treatment of cells with 0.3 IU/ml FSH or rapamycin (*P < 0.05, compared with the control). Please note different y-axis scales in the graphs. Reproductive BioMedicine Online 2017 35, 485-493DOI: (10.1016/j.rbmo.2017.07.016) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

Figure 5 SK6 protein expression levels in human granulosa cells, following the treatments of cells with rapamycin. Fold change in SK6 levels compared with that in the control (P = 0.0313). *P ≤ 0.05, compared with the control. Reproductive BioMedicine Online 2017 35, 485-493DOI: (10.1016/j.rbmo.2017.07.016) Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions