Endurable injectable mesenchymal stem cell therapy for osteoarthritis by encapsulation in alginate constructs M.J. Leijs, G.M. van Buul, M.F. Nieboer,

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Endurable injectable mesenchymal stem cell therapy for osteoarthritis by encapsulation in alginate constructs M.J. Leijs, G.M. van Buul, M.F. Nieboer, J.C. Haeck, N. Kops, P.K. Bos, J.A. Verhaar, M.R. Bernsen, G.J. van Osch Osteoarthritis and Cartilage Volume 24, Pages S12-S13 (April 2016) DOI: 10.1016/j.joca.2016.01.053 Copyright © 2016 Terms and Conditions

Figure 1 Survival and function of encapsulated MSCs in high M and high G alginate for two weeks in vitro. (A) MSC survival measured by CyQuant DNA assay. (B) Viable MSC count in LIVE/DEAD® assay. (C) Immunomodulatory IDO activity of activated MSCs with inflammatory factors. (D) Secretion of immunomodulatory factor IL-6 by activated MSCs with inflammatory factors. Osteoarthritis and Cartilage 2016 24, S12-S13DOI: (10.1016/j.joca.2016.01.053) Copyright © 2016 Terms and Conditions

Figure 2 Localisation and effect of encapsulated allogeneic MSCs in vivo for one month. (A) Construct localisation by MRI directly after implantation (T = 0). (B) Construct localisation by MRI at 1 month. (C) PERL's iron + CD68 immuno-histochemistry of the alginate construct 1 month after implantation (blue = SPIO label MSCs). (D) PERL's iron + CD68 immunohistochemistry of the s.c. pocket1 month after implantation (blue = SPIO label MSCs; pink = macrophage). Osteoarthritis and Cartilage 2016 24, S12-S13DOI: (10.1016/j.joca.2016.01.053) Copyright © 2016 Terms and Conditions

Figure 3 Integrity and development of the construct for intra-articular use. (A,B) Construct integrity follow up by MRI of gadolinium crosslinked constructs over 1 month. (C) 1.1% high G alginate microbeads crosslinked with gadolinium and localized by MRI after infra-articular injection in a rat knee. Osteoarthritis and Cartilage 2016 24, S12-S13DOI: (10.1016/j.joca.2016.01.053) Copyright © 2016 Terms and Conditions