Genetic Analysis of Male Pattern Baldness and the 5α-Reductase Genes Justine A. Ellis, Margaret Stebbing, Stephen B. Harrap  Journal of Investigative Dermatology  Volume 110, Issue 6, Pages 849-853 (June 1998) DOI: 10.1046/j.1523-1747.1998.00224.x Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 DNA fragment sizes detected with HinfI andNspI RFLPs of the 5α-reductase type I gene. (a) Fragment sizes for the HinfI RFLP. The uncut (“A”) allele is indicated by a fragment of ≈210 bp, while the cut (“B”) allele is indicated by two fragments of 138bp and 72bp. Lane 1, heterozygote for the “A” and “B” alleles; lane 2, homozygote for the “A” allele; lane 3, homozygote for the “B” allele. (b) Fragment sizes for the NspI RFLP. The uncut (“a”) allele is indicated by a fragment of ≈288bp, while the cut (“b”) allele is indicated by two fragments of 151bp and 137bp. Lane 1, homozygote for the “b” allele; lane 2, heterozygote for the “a” and “b” alleles; lane 3, heterozygote for the “a” and “b” alleles; lane 4, homozygote for the “a” allele. Journal of Investigative Dermatology 1998 110, 849-853DOI: (10.1046/j.1523-1747.1998.00224.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 DNA fragment sizes detected with the RsaI RFLP of the 5α-reductase type II gene. The allele lacking the polymorphic restriction site, termed allele “A,” is indicated by two fragments of 256bp and 93bp. The allele containing the polymorphic restriction site, termed allele “B,” is indicated by three fragments of 236 bp, 93 bp, and 20bp (the 20bp fragment could not be clearly resolved). Lane 1, homozygote for the “A” allele; lane 2, heterozygote for the “A” and “B” alleles; lane 3, homozygote for the “B” allele. Journal of Investigative Dermatology 1998 110, 849-853DOI: (10.1046/j.1523-1747.1998.00224.x) Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions