A. Simon, DrMeda, M. Wilhelmia, G. Steinhoff, ProfDrMedb, W

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Presentation transcript:

Cardiac valve endothelial cells: Relevance in the long-term function of biologic valve prostheses A. Simon, DrMeda, M. Wilhelmia, G. Steinhoff, ProfDrMedb, W. Harringer, DrMedb, P. Brücke, ProfDrMedc, A. Haverich, ProfDrMedb The Journal of Thoracic and Cardiovascular Surgery Volume 116, Issue 4, Pages 609-616 (October 1998) DOI: 10.1016/S0022-5223(98)70167-9 Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 1 HCVE in vitro (APAAP stain, original magnification ×700). Confluent monolayer of valvular endothelium in in vitro culture. Cells are stained for MHC class I antigens. Typical appearance, showing that cells are polygonal and grow as confluent monolayer without detectable growth pattern. Cell borders are not identifiable. Nuclei are central with 1 to 3 nucleoli. The Journal of Thoracic and Cardiovascular Surgery 1998 116, 609-616DOI: (10.1016/S0022-5223(98)70167-9) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 2 Cryostat sections. A, Reference aortic valve (APAAP stain, original magnification ×400). Typical appearance of endothelial layer on “normal valves” and valves from previously transplanted hearts. Valve is stained for expression of CD31 antigens, showing complete endothelial lining. Expression of CD31 is restricted to the endothelial cells. Internal tissue was well preserved, showing no signs of calcification or degeneration. B, Explanted allograft valve prosthesis (POD stain, original magnification ×200). Typical appearance of explanted allograft valve, showing denuded surface and breakup of internal structure. The Journal of Thoracic and Cardiovascular Surgery 1998 116, 609-616DOI: (10.1016/S0022-5223(98)70167-9) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 4 Cryostat section (APAAP stain, original magnification ×400). Immunohistochemical staining (anti-CD31) of valve obtained at time of retransplantation, showing a continuous monolayer of positive cells on the surface. The internal structure is well preserved. The Journal of Thoracic and Cardiovascular Surgery 1998 116, 609-616DOI: (10.1016/S0022-5223(98)70167-9) Copyright © 1998 Mosby, Inc. Terms and Conditions

Fig. 3 Cryostat sections. Immunohistochemical analysis of sections of explanted aortic valve allograft. A, Leaflet section stained for CD54 antigens, showing partial re-endothelialization. A positive monolayer of cells can be detected on the valvular surface. In other areas of the valve, infiltrating CD54-positive cells could be demonstrated (not shown). The internal structure is partially preserved, although breakup and calcification could be demonstrated. (APAAP stain, original magnification ×400.) B, Leaflet section stained for CD62P antigens, showing partial re-endothelialization. A monolayer of cells on the valvular surface can be detected. Staining is contained to the valvular surface. (APAAP stain, original magnification ×100.) C, Leaflet section stained for CD54 antigens, again showing partial re-endothelialization. As with CD62P, the staining is contained to the monolayer of cells on the valvular surface. (APAAP stain, original magnification ×400.) The Journal of Thoracic and Cardiovascular Surgery 1998 116, 609-616DOI: (10.1016/S0022-5223(98)70167-9) Copyright © 1998 Mosby, Inc. Terms and Conditions