Association of the blaCMY-10 gene with a novel complex class 1 integron carrying an ISCR1 element in clinical isolates from Korea J.S. Song, S.J. Jang,

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

The first report of detecting the blaSIM-2 gene and determining the complete sequence of the SIM-encoding plasmid F. Sun, D. Zhou, Q. Wang, J. Feng,

E. Bapteste, C. Bicep, P. Lopez Clinical Microbiology and Infection

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections L. Cnops, J. Jacobs, M. Van Esbroeck Clinical.

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

Characterisation of OXA-51, a novel class D carbapenemase found in genetically unrelated clinical strains of Acinetobacter baumannii from Argentina S.

Distribution of mef(A)-containing genetic elements in erythromycin-resistant isolates of Streptococcus pyogenes from Italy S.D. Ercole, D. Petrelli,

Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China Z. Xu, L. Shi, C. Zhang, L. Zhang, X. Li,

Salmonella genomic island 1-J variants associated with change in the antibiotic resistance gene cluster in multidrug-resistant Salmonella enterica serovar.

Migrant health—a cause for concern?

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

A.K. Reddy, P. Garg, I. Kaur Clinical Microbiology and Infection

O. Barraud, M. Casellas, C. Dagot, M.-C. Ploy

B.J. Kocjan, D. Bzhalava, O. Forslund, J. Dillner, M. Poljak

A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU P.G. Higgins, H. Wisplinghoff, O. Krut, H.

A novel VIM‐type metallo‐beta‐lactamase (VIM‐14) in a Pseudomonas aeruginosa clinical isolate from a neonatal intensive care unit A. Mazzariol, C. Mammina,

B.J. Kocjan, D. Bzhalava, O. Forslund, J. Dillner, M. Poljak

Evaluation of differential gene expression in susceptible and resistant clinical isolates of Klebsiella pneumoniae by DNA microarray analysis A. Doménech-Sánchez,

Genetic association of blaSHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan W.L. Yu, S.C. Chen, S.W. Hung, Y.C. Chuang,

High prevalence of ST-78 infection-associated vancomycin-resistant Enterococcus faecium from hospitals in Asunción, Paraguay M.A. Khan, J.B. Northwood,

Bacteriophages as a reservoir of extended-spectrum β -lactamase and fluoroquinolone resistance genes in the environment E. Marti, E. Variatza, J.L. Balcázar

Chromosomal integration of the novel plasmid pUR3912 from methicillin-susceptible Staphylococcus aureus ST398 of human origin E. Gómez-Sanz, M. Zarazaga,

Isolation and characterisation of toxin A-negative, toxin B-positive Clostridium difficile in Dublin, Ireland D. Drudy, N. Harnedy, S. Fanning, R. O'Mahony,

Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China Z. Xu, L. Shi, C. Zhang, L. Zhang, X. Li,

Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays C. Putaporntip, P.

Vector control: a cornerstone in the malaria elimination campaign

Genetic characterisation of Helicobacter pylori isolates from an Argentinean adult population based on cag pathogenicity island right-end motifs, lspA-glmM.

Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for.

Novel and uncommon antimicrobial resistance genes in livestock-associated methicillin- resistant Staphylococcus aureus K. Kadlec, A.T. Feßler, T. Hauschild,

The first report of detecting the blaSIM-2 gene and determining the complete sequence of the SIM-encoding plasmid F. Sun, D. Zhou, Q. Wang, J. Feng,

New Delhi metallo-beta-lactamase (NDM-1): towards a new pandemia?

Emerging carbapenemases in Gram-negative aerobes

Comparison of computed tomographic findings in pulmonary mucormycosis and invasive pulmonary aspergillosis J. Jung, M.Y. Kim, H.J. Lee, Y.S. Park, S.-O.

Training for the infectious diseases speciality in Norway

Dissemination of multidrug-resistant, class 1 integron-carrying Acinetobacter baumannii isolates in Taiwan L.-Y. Huang, T.-L. Chen, P.-L. Lu, C.-A. Tsai,

Occurrence of extended-spectrum β-lactamase-producing Salmonella enterica in northern Spain with evidence of CTX-M-9 clonal spread among animals and humans

Integrons and gene cassettes in clinical isolates of co-trimoxazole-resistant Gram- negative bacteria M. Grape, A. Farra, G. Kronvall, L. Sundström Clinical.

CMI editorial report 2011 Clinical Microbiology and Infection

S. A. Gomez, F. G. Pasteran, D. Faccone, N. Tijet, M. Rapoport, C

E. Bapteste, C. Bicep, P. Lopez Clinical Microbiology and Infection

R. A. Bonnin, L. Poirel, T. Naas, M. Pirs, K. Seme, J. Schrenzel, P

Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China J. Sun, M. Zhou, Q. Wu, Y.

C. Héritier, L. Poirel, P. Nordmann

Metagenomics and probiotics

A highly sensitive novel PCR assay for detection of Pneumocystis jirovecii DNA in bronchoalveloar lavage specimens from immunocompromised patients T.

Association of blaDHA-1 and qnrB genes carried by broad-host-range plasmids among isolates of Enterobacteriaceae at a Spanish hospital C. Mata, E. Miró,

A. Sundsfjord, G. Skov Simonsen, P. Courvalin

Nosocomial listeria gastroenteritis in a newborn, confirmed by random amplification of polymorphic DNA H. Hof, R. Lampidis, J. Bensch Clinical Microbiology.

Increased incidence of methicillin-resistant Staphylococcus aureus ST80, novel ST125 and SCCmecIV in the south-eastern part of Norway during a 12-year.

Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli A. Alperi,

Accurate hepatitis C virus genotyping and selection of optimal therapy: lessons from a St Petersburg strain infection E. Knops, E. Heger Clinical Microbiology.

A.P. Underwood, J. Green Clinical Microbiology and Infection

Rebecca J. Seward, Kevin J. Towner Clinical Microbiology and Infection

Clinical Microbiology and Infection

I. Bitar, A. Piazza Clinical Microbiology and Infection

Comparison of one-tube multiplex PCR, automated ribotyping and intergenic spacer (ITS) sequencing for rapid identification of Acinetobacter baumannii

Molecular epidemiology and genetic diversity of human astrovirus in South Korea from 2002 to 2007 A.Y. Jeong, H.S. Jeong, M.Y. Jo, S.Y. Jung, M.S. Lee,

U. Garza-Ramos, G. Davila, V. Gonzalez, C. Alpuche-Aranda, V. R

Resistance integrons and super-integrons

High-resolution melting analysis for the detection of two erythromycin-resistant Bordetella pertussis strains carried by healthy schoolchildren in China

J.J. Keller, M.-C. Tsai, C.-C. Lin, Y.-C. Lin, H.-C. Lin

Detection of Bartonella henselae and Bartonella quintana by a simple and rapid procedure using broad-range PCR amplification and direct single-strand.

Modelling during an emergency: the 2009 H1N1 influenza pandemic

K. S. Ko, T. Kuwahara, L. Haehwa, Y. -J. Yoon, B. -J. Kim, K. -H

J.L. Balcázar Clinical Microbiology and Infection

Typing of Clostridium difficile

L. Poirel, T. Vu Nguyen, A. Weintraub, C. Leviandier, P. Nordmann

The future of diagnostic bacteriology

The origin of a methicillin-resistant Staphylococcus aureus isolate at a neonatal ward in Sweden—possible horizontal transfer of a staphylococcal cassette.

Presentation transcript:

Association of the blaCMY-10 gene with a novel complex class 1 integron carrying an ISCR1 element in clinical isolates from Korea J.S. Song, S.J. Jang, J.J. Lee, J.H. Lee, I.K. Bae, B.C. Jeong, S.-S. Cha, J.-H. Lee, S.-K. Hong, S.H. Lee Clinical Microbiology and Infection Volume 16, Issue 7, Pages 1013-1017 (July 2010) DOI: 10.1111/j.1469-0691.2009.03002.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 (a) Schematic representation of the backbone structure of the complex class 1 integron harbouring novel genes on a 130-kbp conjugative plasmid from an Enterobacter aerogenes isolate. Overlapping PCR amplifications and DNA sequencing with specific primers (Table 1) revealed that the new integron was identical in the remaining clinical isolates. Open arrow, open reading frames; white oval, 59-be element; black oval, attI1 recombination site; Pc (5′-TGGACATAAGCCTGTTCGGTTCGTAAACT-3’) and Pint (5′-AGTCTATGCCTCGGGCATCCAAGCAGCAA-3’) are promoters for cassette genes and the integrase (intI1) gene, respectively. The −35 and −10 motifs of the blaCMY-10 gene promoter are boxed. The recombination cross-over site is underlined. oriIS (the initiation site of ISCR1 transposition) is boxed and underlined. qacEΔ1(140) shows a 140-bp deletion at the 5′-end of qacEΔ1, and the remainder of the two genes are identical. (b) The three subclones used for sequencing. (c) Comparison of the upstream nucleotide sequences of four plasmid-mediated blaCMY β-lactamase genes. Sequence identity is indicated by asterisks. The absence of some nucleotides is indicated by dots. The recombination cross-over site (RCS) and oriIS are underlined and boxed, respectively. The additional 15 bases described in this study are heavily underlined. The start codon of the blaCMY genes is bold and heavily underlined. GenBank accession numbers are as follows: blaCMY-1, X92508; blaCMY-8, EF382672; blaCMY-9, AB061794; blaCMY-10, FJ004895. Clinical Microbiology and Infection 2010 16, 1013-1017DOI: (10.1111/j.1469-0691.2009.03002.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions