Katie S Kindt, Tobey Tam, Shaleah Whiteman, William R Schafer 

Slides:



Advertisements
Similar presentations
Volume 30, Issue 5, Pages (September 2014)
Advertisements

Volume 17, Issue 7, Pages (April 2007)
Volume 19, Issue 4, Pages (April 2017)
Mary Sym, Naomi Robinson, Cynthia Kenyon  Cell 
Hox Genes Promote Neuronal Subtype Diversification through Posterior Induction in Caenorhabditis elegans  Chaogu Zheng, Margarete Diaz-Cuadros, Martin.
C. elegans Class B Synthetic Multivulva Genes Act in G1 Regulation
Volume 19, Issue 4, Pages (April 2017)
Volume 14, Issue 1, Pages (January 2008)
Anneliese M. Schaefer, Gayla D. Hadwiger, Michael L. Nonet  Neuron 
Differential Functions of the C
Volume 21, Issue 11, Pages (June 2011)
Volume 30, Issue 5, Pages (September 2014)
Volume 79, Issue 2, Pages (July 2013)
Tissue-Specific Activities of C
Volume 2, Issue 3, Pages (March 2002)
Cellular Stress Induces a Protective Sleep-like State in C. elegans
Volume 30, Issue 5, Pages (September 2014)
Massimo A. Hilliard, Cornelia I. Bargmann, Paolo Bazzicalupo 
Regulation of Presynaptic Terminal Organization by C
Volume 2, Issue 3, Pages (March 2002)
An Integrated Serotonin and Octopamine Neuronal Circuit Directs the Release of an Endocrine Signal to Control C. elegans Body Fat  Tallie Noble, Jonathan.
Ilan D Zipkin, Rachel M Kindt, Cynthia J Kenyon  Cell 
Paul D Baum, Gian Garriga  Neuron  Volume 19, Issue 1, Pages (July 1997)
Jennifer Whangbo, Cynthia Kenyon  Molecular Cell 
Volume 55, Issue 4, Pages (August 2007)
Volume 15, Issue 24, Pages (December 2005)
Volume 9, Issue 3, Pages (September 2005)
Volume 43, Issue 2, Pages e7 (October 2017)
The Conserved Immunoglobulin Superfamily Member SAX-3/Robo Directs Multiple Aspects of Axon Guidance in C. elegans  Jennifer A Zallen, B.Alexander Yi,
Anterior-Posterior Gradient in Neural Stem and Daughter Cell Proliferation Governed by Spatial and Temporal Hox Control  Ignacio Monedero Cobeta, Behzad.
Volume 11, Issue 17, Pages (September 2001)
Chaogu Zheng, Felix Qiaochu Jin, Martin Chalfie  Cell Reports 
POP-1 and Anterior–Posterior Fate Decisions in C. elegans Embryos
Serotonin Inhibition of Synaptic Transmission
An AP2 Transcription Factor Is Required for a Sleep-Active Neuron to Induce Sleep-like Quiescence in C. elegans  Michal Turek, Ines Lewandrowski, Henrik.
Opposing Wnt Pathways Orient Cell Polarity during Organogenesis
Volume 118, Issue 6, Pages (September 2004)
Anchor Cell Invasion into the Vulval Epithelium in C. elegans
Volume 15, Issue 15, Pages (August 2005)
Volume 79, Issue 2, Pages (July 2013)
Massimo A. Hilliard, Cornelia I. Bargmann  Developmental Cell 
An AP2 Transcription Factor Is Required for a Sleep-Active Neuron to Induce Sleep-like Quiescence in C. elegans  Michal Turek, Ines Lewandrowski, Henrik.
Lateral Facilitation between Primary Mechanosensory Neurons Controls Nose Touch Perception in C. elegans  Marios Chatzigeorgiou, William R. Schafer  Neuron 
Volume 16, Issue 9, Pages (May 2006)
Volume 71, Issue 1, Pages (July 2011)
Volume 10, Issue 3, Pages (March 2006)
Rapid Assembly of Presynaptic Materials behind the Growth Cone in Dopaminergic Neurons Is Mediated by Precise Regulation of Axonal Transport  David M.
Volume 11, Issue 11, Pages (June 2015)
Hitoshi Sawa, Hiroko Kouike, Hideyuki Okano  Molecular Cell 
The C. elegans evl-20 Gene Is a Homolog of the Small GTPase ARL2 and Regulates Cytoskeleton Dynamics during Cytokinesis and Morphogenesis  Igor Antoshechkin,
MAX-1, a Novel PH/MyTH4/FERM Domain Cytoplasmic Protein Implicated in Netrin- Mediated Axon Repulsion  Xun Huang, Hwai-Jong Cheng, Marc Tessier-Lavigne,
Volume 15, Issue 15, Pages (August 2005)
The CMK-1 CaMKI and the TAX-4 Cyclic Nucleotide-Gated Channel Regulate Thermosensory Neuron Gene Expression and Function in C. elegans  John S. Satterlee,
Volume 87, Issue 2, Pages (October 1996)
Volume 32, Issue 1, Pages (October 2001)
Islet Coordinately Regulates Motor Axon Guidance and Dendrite Targeting through the Frazzled/DCC Receptor  Celine Santiago, Greg J. Bashaw  Cell Reports 
Interaxonal Interaction Defines Tiled Presynaptic Innervation in C
Volume 109, Issue 5, Pages (May 2002)
Hulusi Cinar, Sunduz Keles, Yishi Jin  Current Biology 
Numb Antagonizes Notch Signaling to Specify Sibling Neuron Cell Fates
The C. elegans evl-20 Gene Is a Homolog of the Small GTPase ARL2 and Regulates Cytoskeleton Dynamics during Cytokinesis and Morphogenesis  Igor Antoshechkin,
Fig. 5. EGL-20 inhibits anterior and posterior orientation of UNC-40 asymmetric localization and the formation of axons from these sites.(A–D) HSN neurons.
Christopher C. Quinn, Douglas S. Pfeil, William G. Wadsworth 
Volume 15, Issue 17, Pages (September 2005)
Volume 55, Issue 4, Pages (August 2007)
Developmental Timing in C
Brent Neumann, Massimo A. Hilliard  Cell Reports 
Volume 32, Issue 1, Pages (October 2001)
Volume 15, Issue 24, Pages (December 2005)
Volume 18, Issue 6, Pages (June 2010)
Presentation transcript:

Serotonin Promotes Go-Dependent Neuronal Migration in Caenorhabditis elegans  Katie S Kindt, Tobey Tam, Shaleah Whiteman, William R Schafer  Current Biology  Volume 12, Issue 20, Pages 1738-1747 (October 2002) DOI: 10.1016/S0960-9822(02)01199-5

Figure 1 Neuronal Migrations in Wild-Type C. elegans (A) Embryonic migrations. ALML/R and BDUL/R are generated by mitotic divisions of the AB.arppaapp and AB.arpppapp neuroblasts in the embryonic head. In each case, during late embryogenesis, the anterior daughter (ALM) undergoes a long posterior and dorsal migration, while the posterior daughter (BDU) undergoes a shorter posterior migration. (B) QR and QL lineages. During the L1/L2 molt, QR migrates anteriorly, undergoing two divisions to generate the QR.pa neuroblast. QR.pa divides to produce AVM, a touch neuron, and SDQR, an interneuron. In contrast, QL migrates posteriorly, undergoing divisions that produce the QL.pa neuroblast. QL.pa divides to produce PVM, a touch neuron, and SDQL, an interneuron. (C) Postembryonic migrations of the Q descendant neurons. After AVM and SDQR arise from division of QR.pa, SDQR migrates dorsally and anteriorly and projects an axon into the sublateral nerve. AVM migrates ventrally and projects an axon into the ventral nerve cord. QL.pa produces PVM and SDQL, whose cell bodies do not undergo any further migrations. Current Biology 2002 12, 1738-1747DOI: (10.1016/S0960-9822(02)01199-5)

Figure 2 Migration Defects of Serotonin-Deficient Mutants The final positions of SDQR, AVM, BDU, and ALM in tph-1 mutants are summarized. Left is anterior; top is dorsal. Wild-type positions are depicted by darkened circles; lightly shaded circles indicate positions observed after defective migration. The arrows indicate the normal path of migration in wild-type animals, and the percentages indicate the fraction of the cell attaining the position indicated. Current Biology 2002 12, 1738-1747DOI: (10.1016/S0960-9822(02)01199-5)

Figure 3 Analysis of AVM and SDQR Cell Lineages in tph-1 Mutants Representative cell lineages seen in 20 tph-1 mutant animals are shown. The position of QR.pa at the time it divided to produce SDQR and AVM is indicated by the gray box. The positions to which SDQR and AVM migrated are indicated by asterisks. Current Biology 2002 12, 1738-1747DOI: (10.1016/S0960-9822(02)01199-5)

Figure 4 Effect of Exogenous Serotonin on AVM and PVM Migration (A) Rescue of AVM migration defects by exogenous serotonin. Serotonin-deficient animals were allowed to develop on exogenous serotonin (7.5 mM 5-HT) to test whether serotonin could rescue the migration defects of SDQR and AVM. The numbers of animals tested (no drug/on serotonin) were: tph-1, n = 100/n = 50; cat-4, n = 100/n = 50; bas-1, n = 86/n = 60; cat-1, n = 50/n = 50; cat-2, n = 50/n = 100. An asterisk indicates a statistically significant difference between treated and untreated animals. (B–D) Effect of exogenous serotonin on the positioning of PVM. Representative images of wild-type animals expressing pmec-4::GFP in the (B) absence or (C and D) presence of exogenous serotonin are shown. In all images, right is posterior and top is dorsal. The positions of PVD, PDE, and SDQL (and the normal position of PVM) as determined by Nomarski optics are circled. (E) Frequency of serotonin-induced mispositioning of PVM. The normal position of PVM within the postdereid is indicated in bold. The percentage of animals with PVM found at other positions is indicated. A total of 66 animals were tested. Current Biology 2002 12, 1738-1747DOI: (10.1016/S0960-9822(02)01199-5)

Figure 5 Misplacement Phenotypes of goa-1, egl-30, and unc-2 Mutant Animals (A) The positions adopted by ALM, BDU, AVM, and SDQR in egl-30(n686), goa-1(n1134), and unc-2(mu74) mutant animals are summarized (n =100 for egl-30; n = 200 for unc-2 and goa-1). Left is anterior; up is dorsal. Wild-type positions are indicated by darkened circles. Arrows represent embryonic migrations to wild-type position for ALM and BDU, and migration from the final division of QR.pa into wild-type positions of AVM and SDQR. (B) AVM and SDQR migration defects are not rescued by exogenous serotonin in goa-1(n1134) and unc-2(mu74) mutants. Mutant animals were allowed to develop on exogenous serotonin (7.5 mM 5-HT). The numbers of animals tested (no drug/on serotonin) were: goa-1(n1134), n = 200/n = 100; unc-2(mu74), n = 200/n = 100. Current Biology 2002 12, 1738-1747DOI: (10.1016/S0960-9822(02)01199-5)