The Stochastic Search Dynamics of Interneuron Migration

Slides:



Advertisements
Similar presentations
Nonlinear Poisson Equation for Heterogeneous Media Langhua Hu, Guo-Wei Wei Biophysical Journal Volume 103, Issue 4, Pages (August 2012) DOI: /j.bpj
Advertisements

Pressure and Temperature Dependence of Growth and Morphology of Escherichia coli: Experiments and Stochastic Model  Pradeep Kumar, Albert Libchaber  Biophysical.
Cortical Microtubule Contacts Position the Spindle in C
Force-Clamp Analysis Techniques Give Highest Rank to Stretched Exponential Unfolding Kinetics in Ubiquitin  Herbert Lannon, Eric Vanden-Eijnden, J. Brujic 
Rapid Assembly of a Multimeric Membrane Protein Pore
Precision and Variability in Bacterial Temperature Sensing
Volume 104, Issue 2, Pages (January 2013)
Bipedal Locomotion in Crawling Cells
Volume 96, Issue 3, Pages (February 2009)
Transcription Stochasticity of Complex Gene Regulation Models
Volume 5, Issue 6, Pages (December 2013)
Volume 112, Issue 6, Pages (March 2017)
The Transfer Functions of Cardiac Tissue during Stochastic Pacing
Lara Scharrel, Rui Ma, René Schneider, Frank Jülicher, Stefan Diez 
Dynamics of the Serine Chemoreceptor in the Escherichia coli Inner Membrane: A High- Speed Single-Molecule Tracking Study  Dongmyung Oh, Yang Yu, Hochan.
Modeling Endoplasmic Reticulum Network Maintenance in a Plant Cell
Susanne Karsch, Deqing Kong, Jörg Großhans, Andreas Janshoff 
Joseph M. Johnson, William J. Betz  Biophysical Journal 
Self-Organization of Myosin II in Reconstituted Actomyosin Bundles
Volume 111, Issue 2, Pages (July 2016)
Hirokazu Tanimoto, Masaki Sano  Biophysical Journal 
Is Aggregate-Dependent Yeast Aging Fortuitous
Single Vesicle Assaying of SNARE-Synaptotagmin-Driven Fusion Reveals Fast and Slow Modes of Both Docking and Fusion and Intrasample Heterogeneity  Sune.
Rainer Kurre, Berenike Maier  Biophysical Journal 
Cellular Contraction Can Drive Rapid Epithelial Flows
Homodimeric Kinesin-2 KIF3CC Promotes Microtubule Dynamics
Cholesterol Depletion Mimics the Effect of Cytoskeletal Destabilization on Membrane Dynamics of the Serotonin1A Receptor: A zFCS Study  Sourav Ganguly,
Qiaochu Li, Stephen J. King, Ajay Gopinathan, Jing Xu 
Volume 109, Issue 12, Pages (December 2015)
H.M. Seeger, G. Marino, A. Alessandrini, P. Facci  Biophysical Journal 
V.M. Burlakov, R. Taylor, J. Koerner, N. Emptage  Biophysical Journal 
Volume 103, Issue 10, Pages (November 2012)
Volume 107, Issue 7, Pages (October 2014)
Martin Clausen, Michael Koomey, Berenike Maier  Biophysical Journal 
Actin Assembly at Model-Supported Lipid Bilayers
Real-Time Nanopore-Based Recognition of Protein Translocation Success
Volume 111, Issue 12, Pages (December 2016)
Substrate Deformation Predicts Neuronal Growth Cone Advance
Guidance of Cell Migration by Substrate Dimension
Quantitative Image Restoration in Bright Field Optical Microscopy
Rapid Assembly of a Multimeric Membrane Protein Pore
Stochastic Pacing Inhibits Spatially Discordant Cardiac Alternans
Volume 110, Issue 7, Pages (April 2016)
Volume 84, Issue 1, Pages (January 2003)
Volume 99, Issue 8, Pages (October 2010)
Satomi Matsuoka, Tatsuo Shibata, Masahiro Ueda  Biophysical Journal 
Aligning Paramecium caudatum with Static Magnetic Fields
Chemically Mediated Mechanical Expansion of the Pollen Tube Cell Wall
Directional Persistence of Cell Migration Coincides with Stability of Asymmetric Intracellular Signaling  Michael C. Weiger, Shoeb Ahmed, Erik S. Welf,
Andrew E. Blanchard, Chen Liao, Ting Lu  Biophysical Journal 
Volume 101, Issue 7, Pages (October 2011)
Volume 90, Issue 10, Pages (May 2006)
Volume 108, Issue 10, Pages (May 2015)
Dynamics of Mouth Opening in Hydra
Long-Range Nonanomalous Diffusion of Quantum Dot-Labeled Aquaporin-1 Water Channels in the Cell Plasma Membrane  Jonathan M. Crane, A.S. Verkman  Biophysical.
Volume 97, Issue 7, Pages (October 2009)
Abir M. Kabbani, Christopher V. Kelly  Biophysical Journal 
Interaction of Oxazole Yellow Dyes with DNA Studied with Hybrid Optical Tweezers and Fluorescence Microscopy  C.U. Murade, V. Subramaniam, C. Otto, Martin.
Stochastic Pacing Inhibits Spatially Discordant Cardiac Alternans
Localization Precision in Stepwise Photobleaching Experiments
Christina Ketchum, Heather Miller, Wenxia Song, Arpita Upadhyaya 
Volume 113, Issue 3, Pages (August 2017)
Modeling Endoplasmic Reticulum Network Maintenance in a Plant Cell
Volume 107, Issue 3, Pages (August 2014)
Volume 113, Issue 10, Pages (November 2017)
Volume 97, Issue 1, Pages (July 2009)
Bekele Gurmessa, Shea Ricketts, Rae M. Robertson-Anderson 
Volume 15, Issue 9, Pages (May 2005)
Volume 101, Issue 7, Pages (October 2011)
George D. Dickinson, Ian Parker  Biophysical Journal 
Presentation transcript:

The Stochastic Search Dynamics of Interneuron Migration Joanne M. Britto, Leigh A. Johnston, Seong-Seng Tan  Biophysical Journal  Volume 97, Issue 3, Pages 699-709 (August 2009) DOI: 10.1016/j.bpj.2009.04.064 Copyright © 2009 Biophysical Society Terms and Conditions

Figure 1 Migratory behaviors of cortical interneurons. (A) Schematic depicting migration of interneurons from the medial ganglionic eminence (MGE) into the cortex. Organotypic slices of E12.5 GAD67GFP/+ cortex at (B) 4 h and (C) 24 h in culture. (D) Images of control, (E) BDNF, and (F) blebbistatin-treated interneurons show changes in cellular morphology. (G) Time-lapse sequence of migrating interneuron taken at 5-min intervals. (H) Schematic of tracked time-lapse sequence showing nucleus (black circle), branches (color circle), and branch points (black square). Each branch is assigned a color to represent the hierarchy of origin. Scale bars: (B and C) 200μm; (D–G) 20 μm. Biophysical Journal 2009 97, 699-709DOI: (10.1016/j.bpj.2009.04.064) Copyright © 2009 Biophysical Society Terms and Conditions

Figure 2 Stochastic dynamics of neurite branching in control interneurons. (A) Histogram of branch interarrival times and best-fit exponential pdfs (Eq. 1, solid lines). (B) Histogram of branch lifespans and best-fit exponential pdfs (Eq. 2, solid lines). (C) Histogram of neuritic arbor size overlaid by empirically derived shifted Poisson distribution (Eq. 10, solid line) parameterized by ρe, corresponding to the model in panel I. Hypothesized models of neuritic arbor dynamics: (D) ordained branch retraction model in which only one branch is available for the next retraction (equivalently a single-server queue). (E) No steady-state distribution exists as λ/μ > 1 for control interneurons (see Table 1). (F) Unrestricted branch retraction model (equivalently a multiserver queue). (G) Histogram of neuritic arbor size overlaid by steady-state Poisson distribution (Eq. 8, solid line) parameterized by ρˆ. (H) Restricted branch retraction model, based on the observation that migrating interneurons always display one or more branches. (I) Histogram of neuritic arbor size overlaid by steady-state-shifted Poisson distribution (Eq. 10, solid line) parameterized by ρm (Eq. 11). Biophysical Journal 2009 97, 699-709DOI: (10.1016/j.bpj.2009.04.064) Copyright © 2009 Biophysical Society Terms and Conditions

Figure 3 Birth-death model results for neurite branch dynamics in the presence of BDNF and blebbistatin. Histograms of branch interarrival times and best-fit exponential pdfs (Eq. 1, solid lines) for (A) BDNF and (B) blebbistatin. Histograms of branch lifespans and best-fit exponential pdfs (Eq. 2, solid lines) for (C) BDNF and (D) blebbistatin. Histograms of neuritic arbor size overlaid by empirically derived shifted Poisson distributions (Eq. 10, solid lines) parameterized by ρe for (E) BDNF and (F) blebbistatin. Biophysical Journal 2009 97, 699-709DOI: (10.1016/j.bpj.2009.04.064) Copyright © 2009 Biophysical Society Terms and Conditions

Figure 4 Comparison between empirically derived branch populations and model-based predictions. Probability distribution functions (Eq. 10) of average branch numbers derived from model-based and empirically-derived estimates, ρm and ρe, respectively. The BDNF distribution (KS-test, p < 0.0001) and median branch number (KW-test, p < 0.0001) are significantly different to control and blebbistatin. Biophysical Journal 2009 97, 699-709DOI: (10.1016/j.bpj.2009.04.064) Copyright © 2009 Biophysical Society Terms and Conditions

Figure 5 Nucleokinesis dynamics are unperturbed by branching disturbance. Plots show accumulated distance of nucleus movement over time from experimental data (solid line) and model-based predictions (dotted line) for (A) control and (B) BDNF-treated interneurons. Distribution of estimated parameters, k and c, for (C) control and (D) BDNF showing no significant difference between control and treatment (Hotelling's T-square test). Open lines depict critical damping, k =c2/4. Biophysical Journal 2009 97, 699-709DOI: (10.1016/j.bpj.2009.04.064) Copyright © 2009 Biophysical Society Terms and Conditions

Figure 6 Relationship between branch dynamics and nucleokinesis. Histograms and best-fit exponential distributions of time intervals between nucleokinesis onset and branch (A) births and (B) deaths for control interneurons, and (C) births and (D) deaths for BDNF interneurons. Example of (E) a control interneuron and (F) a BDNF-treated interneuron undergoing nucleokinesis. The leading branch in each case (marked by ∗ in first and last frames) grows throughout the nucleokinesis event. Biophysical Journal 2009 97, 699-709DOI: (10.1016/j.bpj.2009.04.064) Copyright © 2009 Biophysical Society Terms and Conditions