Mary C Mahony, Ph. D. , Dave J Swanlund, M. S. , Michelle Billeter, B

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Presentation transcript:

Regional Distribution of 5α-Reductase Type 1 and Type 2 mRNA Along the Human Epididymis Mary C Mahony, Ph.D., Dave J Swanlund, M.S., Michelle Billeter, B.S., Ken P Roberts, Ph.D., Jon L Pryor, M.D. Fertility and Sterility Volume 69, Issue 6, Pages 1116-1121 (June 1998) DOI: 10.1016/S0015-0282(98)00094-6

FIGURE 1 Autoradiograph of a Northern blot analysis of human epididymal RNA. Total RNA (10 μg) isolated from the testis and from the proximal caput (PCp), caput (Cp), distal caput (DCp), corpus (Co), proximal cauda (PCd), and distal cauda (DCd) regions of the human epididymis was subjected to electrophoresis through a denaturing 1.2% agarose gel, transferred, and probed with 5α-reductase type 2 and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) (control) cDNAs. Fertility and Sterility 1998 69, 1116-1121DOI: (10.1016/S0015-0282(98)00094-6)

FIGURE 2 The coamplification of 5α-reductase type 1 (•) (A) and 5α-reductase type 2 (■) (B) mRNA with cyclophilin (▴) mRNA by PCR. Reverse-transcribed mRNA (50 ng) in the human midcaput epididymis was coamplified using a final primer concentration of 20 μM each for either 5α-reductase type 1 or type 2 with cyclophilin primers (10 μM). Products from 24 to 40 cycles were separated by polyacrylamide gel electrophoresis and stained with ethidium bromide. The abundance of PCR products is expressed as the log cpm measured by scintillation spectroscopy from excised bands. Fertility and Sterility 1998 69, 1116-1121DOI: (10.1016/S0015-0282(98)00094-6)

FIGURE 3 Relative abundance of 5α-reductase type 1 (A) and 5α-reductase type 2 (B) mRNA along the human testis and in the proximal caput (PCp), caput (Cp), distal caput (DCp), corpus (Co), proximal cauda (PCd), and distal cauda (DCd) regions of the epididymis. Results are expressed as the means of data obtained from two subjects. Fertility and Sterility 1998 69, 1116-1121DOI: (10.1016/S0015-0282(98)00094-6)