Characterization and mutation analysis of the human FORMIN-2 (FMN2) gene in women with unexplained infertility  David A. Ryley, M.D., Hsin-Hung Wu, M.D., Benjamin Leader, M.D., Alison Zimon, M.D., Richard H. Reindollar, M.D., Mark R. Gray, Ph.D.  Fertility and Sterility  Volume 83, Issue 5, Pages 1363-1371 (May 2005) DOI: 10.1016/j.fertnstert.2004.10.051 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Polymerase chain reaction (PCR) amplification of the FMN2 gene. Twenty-seven fragments that included the 18 FMN2 exons are represented by electrophoretic bands on 1.5% agarose gels stained with ethidium bromide and exposed to ultraviolet light. Fragment 5-L, circled in the top gel, was rich in GC base pairs and appeared as a faint band in 24 of the 69 DNA samples studied, including two of the test subjects. M = HyperLadder electrophoretic marker (Bioline). Fertility and Sterility 2005 83, 1363-1371DOI: (10.1016/j.fertnstert.2004.10.051) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified fragments of the FMN2 gene. Twenty-six DNA fragments were subjected to DGGE. Ten bands were identified on 30%–90% DGGE gels, and 16 bands were identified on 20%–80% DGGE gels. (A) Fragment melting polymorphisms found in 11 of 26 fragments. H = high allele; M = middle allele; L = low allele. (B) *A homozygous fragment melting polymorphism was detected in the exon 9/10 PCR fragment in one of the test subjects and not in any of the 62 control subjects. Fertility and Sterility 2005 83, 1363-1371DOI: (10.1016/j.fertnstert.2004.10.051) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions