Volume 132, Issue 2, Pages 687-697 (February 2007) Hepatic Expression of Candidate Genes in Patients With Alcoholic Hepatitis: Correlation With Disease Severity  Jordi Colmenero, Ramón Bataller, Pau Sancho–Bru, Pablo Bellot, Rosa Miquel, Montserrat Moreno, Pedro Jares, Jaime Bosch, Vicente Arroyo, Joan Caballería, Pere Ginès  Gastroenterology  Volume 132, Issue 2, Pages 687-697 (February 2007) DOI: 10.1053/j.gastro.2006.12.036 Copyright © 2007 AGA Institute Terms and Conditions

Figure 1 Representative photomicrographs of normal human livers and livers from patients with AH stained with (A) Sirius red, (B) α-SMA, and (C) CD43, which are indicative of hepatic fibrosis, myofibroblast accumulation, and infiltration by inflammatory cells, respectively. Original magnification, 200×. (D) Quantification of positive area for Sirius red, α-SMA, and CD43 in all liver specimens using a morphometric method (see Material and Methods section). *P < .001 vs normal livers. Gastroenterology 2007 132, 687-697DOI: (10.1053/j.gastro.2006.12.036) Copyright © 2007 AGA Institute Terms and Conditions

Figure 2 Correlation between the accumulation of (A) myofibroblast cells and (B) inflammatory cells, as assessed by quantification of positive area for α-SMA and CD43 with the degree of fibrosis, as assessed by quantification of positive area for Sirius red. (C) Correlation between the percentage of area with Sirius red staining and the degree of portal hypertension (HVPG). Gastroenterology 2007 132, 687-697DOI: (10.1053/j.gastro.2006.12.036) Copyright © 2007 AGA Institute Terms and Conditions

Figure 3 Correlation between hepatic gene expression and disease severity in patients with AH. Patients with severe AH were defined as those having a Maddrey’s discriminant function of 32 or more. The expression of (A) MMP-2, (B) TIMP-1, (C) GrO-α, and (D) DUOX-1 was significantly higher in patients with severe AH. *P < .001 nonsevere AH vs normal livers. #P < .001 patients with nonsevere AH vs severe AH. Gastroenterology 2007 132, 687-697DOI: (10.1053/j.gastro.2006.12.036) Copyright © 2007 AGA Institute Terms and Conditions

Figure 4 Correlation between hepatic gene expression and histologic findings in patients with AH. (A) GrO-α and (B) DUOX-1 mRNA expression correlated with the finding of severe polymorphonuclear (pmn) infiltrate in liver specimens. *P < .05 vs nonsevere PMN infiltrate. (C) TGFβ-1 and (D) procollagen 4α(I) mRNA expression correlated with the finding of severe lymphocytic infiltrate in liver specimens. *P < .05 vs nonsevere lymphocytic infiltrate. Gastroenterology 2007 132, 687-697DOI: (10.1053/j.gastro.2006.12.036) Copyright © 2007 AGA Institute Terms and Conditions