Yoshiharu Kawaguchi Journal of Investigative Dermatology

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Presentation transcript:

Kit+ Melanocytes Seem to Contribute to Melanocyte Proliferation After UV Exposure as Precursor Cells Yoshiharu Kawaguchi Journal of Investigative Dermatology Volume 116, Issue 6, Pages 920-925 (June 2001) DOI: 10.1046/j.0022-202x.2001.01370.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Melanogenesis caused by UV exposure in C57BL/6 mouse. The mouse was irradiated with 0.18 per cm2 of energy of UVB. Fourteen days after a single exposure, skin samples were dissected and Dopa staining was performed. (a) The epidermal sheet of unirradiated mouse; (b) UV-irradiated mouse. Scale bar: 100 µm. Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Changes of cell populations positive for four melanocyte markers in UV-exposed epidermis. Epidermal sheets were prepared from normal mice (a, d, g, j) and mice at 2 d (b, e, h, k) and 14 d after UV exposure (c, f, i, l), and then stained for TRP-1 (a–c), TRP-2 (d–f), Mitf (g–i), and Kit (j–l). Scale bar: 45 µm. Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Mitf+ and TRP-2+ cells appear before TRP-1+ cells increase after UV exposure. Epidermal sheets were prepared from unirradiated mice (day 0) and mice at 1–14 d after UV exposure, and stained for each melanocyte markers. The numbers of positive cells per mm2 were calculated (mean ± SEM; n = 6). Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Identification of Kit, Mitf double positive cells and Mitf, BrdU double positive cells at 1 d after UV exposure. Epidermal sheets were prepared from mice at 1 and 5 d after single administration of UV exposure. Double immunolabeling were performed as in Materials and Methods. (a) Superimposed image of Kit (green) and Mitf (red). Double positive cells are indicated by arrows. (b) Superimposed image of Mitf (red), BrdU (green), and double positive cells (yellow, arrows) at 1 d. (c) Superimposed image of TRP-1 (green) and BrdU (red) at 5 d. Scale bars: (a) 20 µm; (b) 14 µm; (c) 16 µm. Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 UV irradiation induced high expression of the SCF mRNA in mouse epidermis. Skin specimens were obtained from mouse ear before and 5 h, 1 d, 2 d, and 5 d after UVB irradiation. Total RNA extracted from mouse epidermal sheet was reverse-transcribed using oligo dT as a primer. PCR was performed with the SCF primer set or GAPDH primer. Products of 459 bp DNA represent a soluble form and products of 375 bp DNA a transmembrane form of SCF, respectively. Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 ACK45 attenuated melanogenesis after UV exposure. ACK45 (30 µg per day) was injected to mouse ear skin for 2 d just after UV exposure. Skin samples were dissected at day 2 and day 14 and were subjected to Mitf, TRP-1, or Dopa staining. The numbers of positive cells per mm2 were calculated and then indicated as a percentage for the value of noninjected control mice, respectively (mean ± SEM; n = 5, *p < 0.05). Dopa+ melanocytes in the epidermal sheet of rat IgG-injected mouse (a), and ACK45-injected mouse (b). Relative cell numbers of Mitf+, TRP-1+, and Dopa+ melanocytes in IgG-injected specimen and ACK45-injected specimen (c). Scale bar: 25 µm. Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Proposed process of UV-induced melanogenesis. Journal of Investigative Dermatology 2001 116, 920-925DOI: (10.1046/j.0022-202x.2001.01370.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions