Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium K.H Grinnemo, MD, A Månsson, MD, G Dellgren,

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Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium K.H Grinnemo, MD, A Månsson, MD, G Dellgren,

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Presentation transcript:

Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium K.H Grinnemo, MD, A Månsson, MD, G Dellgren, MD, PhD, D Klingberg, MSc, E Wardell, BSc, V Drvota, MD, PhD, C Tammik, BSc, J Holgersson, MD, PhD, O Ringdén, MD, PhD, C Sylvén, MD, PhD, K Le Blanc, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 127, Issue 5, Pages 1293-1300 (May 2004) DOI: 10.1016/j.jtcvs.2003.07.037

Figure 1 Representative photomicrograph of a transmural scar lesion, 1 week after LAD ligation in an SD rat. (Hematoxylin and eosin, ×10.) The Journal of Thoracic and Cardiovascular Surgery 2004 127, 1293-1300DOI: (10.1016/j.jtcvs.2003.07.037)

Figure 2 Identification of transplanted ahMSC 1 week after transplantation in an RNU rat. In situ hybridization of the chromosomes in the transplanted ahMSC stains red. The Journal of Thoracic and Cardiovascular Surgery 2004 127, 1293-1300DOI: (10.1016/j.jtcvs.2003.07.037)

Figure 3 Responsiveness of SD rat lymphocytes to ahMSC 7 days after injection of ahMSC (B, pooled peripheral blood from 3 syngenic rats; C, 1 individual rat) and with no previous exposure to ahMSC (A), as assayed by MLR. Experiments were performed in triplicate with a significance of P < .01. Mean ± SD. The Journal of Thoracic and Cardiovascular Surgery 2004 127, 1293-1300DOI: (10.1016/j.jtcvs.2003.07.037)

Figure 4 ahMSC elicit a significant proliferative response when cultured with PBL from SD rats but not from RNU rats previously exposed to ahMSC (P < .01). Rat PBL (1 × 105) were cultured with 1 × 105 irradiated autologous lymphocytes in the absence (Bg, Background) or presence (MSC) of 1 × 104 ahMSC. Each bar depicts 1 experiment performed in triplicate. K, control animals; SD, PBL from SD rats 7 days post–cardiac injection of ahMSC; RNU, PBL from an athymic RNU rat 7 days post–cardiac injection of ahMSC. Mean ± SD. The Journal of Thoracic and Cardiovascular Surgery 2004 127, 1293-1300DOI: (10.1016/j.jtcvs.2003.07.037)

Figure 5 Round cell infiltration in the myocardium of normal SD rats: (A) 2 days and (B) 1 week after injection of stem cells and (C) in immunosuppressed SD rats. (Hematoxylin and eosin, ×20.) The Journal of Thoracic and Cardiovascular Surgery 2004 127, 1293-1300DOI: (10.1016/j.jtcvs.2003.07.037)

Figure 6 Immunofluorescence showing infiltration of macrophages (green spots) at the site of injection of stem cells in (A) normal SD rats and (B) RNU rats. The Journal of Thoracic and Cardiovascular Surgery 2004 127, 1293-1300DOI: (10.1016/j.jtcvs.2003.07.037)