Yuji Owada, Ichiro Suzuki, Ryoji Suzuki, Hisatake Kondo

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Altered Water Barrier Function in Epidermal-Type Fatty Acid Binding Protein-Deficient Mice Yuji Owada, Ichiro Suzuki, Ryoji Suzuki, Hisatake Kondo Journal of Investigative Dermatology Volume 118, Issue 3, Pages 430-435 (March 2002) DOI: 10.1046/j.0022-202x.2001.01616.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Target disruption of mouse E-FABP gene. (A) Intron/exon structure of the murine E-FABP gene and representation of the target construct. Homologies (bold line) between targeting construct and genomic DNA, and the probe used for southern blotting are indicated. (B) Southern blot of BamHI-digested skin DNAs from each genotype mouse. (C) Northern blot of total RNA (30 µg) from skins probed with labeled mouse E-FABP cDNA or glyceraldehyde-3-phosphate dehydrogenase cDNA as a loading control. (D) Western blot of skin lysates with antirat E-FABP antibody. 10 µg of protein from skins of each genotype mice were applied. Journal of Investigative Dermatology 2002 118, 430-435DOI: (10.1046/j.0022-202x.2001.01616.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Immunohistochemical analysis of dorsal skin from adult wild-type mice and homozygous mice with E-FABP antibody. (A) Wild-type mice; (B) homozygous mice. Note the absence of positive immunoreactivity throughout the skin of the homozygous mice. Scale bar: 100 μm. Journal of Investigative Dermatology 2002 118, 430-435DOI: (10.1046/j.0022-202x.2001.01616.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Northern bolt analyses of E- and H-FABP in the skin of each genotype mouse. Note the increased expression of H-FABP in the skin of heterozygous and homozygous mice of 0.7 kb. As the internal control, the expression of glyceraldehyde-3-phosphate dehydrogenase mRNA is shown. Journal of Investigative Dermatology 2002 118, 430-435DOI: (10.1046/j.0022-202x.2001.01616.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Chronologic changes of TEWL measured in the dorsal skin of wild-type and homozygous mice following acetone treatment. The time of acetone application is indicated by an arrow; the values at this point represent pretreatment levels. Closed circles, homozygous mice; open squares, wild-type mice. Statistical significance in a paired Student's t test comparing homozygous and wild-type mice is indicated by an asterisk (p < 0.05, n = 6). Journal of Investigative Dermatology 2002 118, 430-435DOI: (10.1046/j.0022-202x.2001.01616.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Electron microscopy of upper portions of the granular cells facing the cornified cells. A substantial number of the lamellar bodies (arrows) are present in the cytoplasm of the granular cells and their exocytotic profiles (*) along the plasma membrane are often seen similarly in both wild (A) and mutant (B) mice without acetone treatment. At 15 min after acetone treatment, the paucity of the lamellar bodies in the cytoplasm of granular cells, but the exocytotic profiles along the plasma membranes, are noticed similarly in both wild (C) and mutant (D) mice. Scale bar: 0.5 μm. Journal of Investigative Dermatology 2002 118, 430-435DOI: (10.1046/j.0022-202x.2001.01616.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions