A Novel Method to Compensate for Different Amplification Efficiencies between Patient DNA Samples in Quantitative Real-Time PCR Jules Meijerink, Caroline.

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A Novel Method to Compensate for Different Amplification Efficiencies between Patient DNA Samples in Quantitative Real-Time PCR Jules Meijerink, Caroline Mandigers, Louis van de Locht, Evelyn Tönnissen, Federico Goodsaid, John Raemaekers The Journal of Molecular Diagnostics Volume 3, Issue 2, Pages 55-61 (May 2001) DOI: 10.1016/S1525-1578(10)60652-6 Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Theoretical schematic illustration of β-actin and albumin amplification curves at high and low reaction efficiencies (E). The β-actin (black curves) and albumin (gray curves) amplification curves and Ct values at high (solid lines) and low (stippled lines) reaction efficiencies for a hypothetical genomic DNA template concentration. Low reaction efficiencies result in higher Ct values for both reaction in a non-linear relationship (Eq. 1). DNA templates with higher efficiencies for both reactions will have lower difference in Ct values between both reference reactions (low ΔCt values) than templates with lower reaction efficiencies (high ΔCt values). The Journal of Molecular Diagnostics 2001 3, 55-61DOI: (10.1016/S1525-1578(10)60652-6) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions