A Novel and Rapid Method of Determining the Effect of Unclassified MLH1 Genetic Variants on Differential Allelic Expression Sheron Perera, Brian Li,

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A Novel and Rapid Method of Determining the Effect of Unclassified MLH1 Genetic Variants on Differential Allelic Expression Sheron Perera, Brian Li, Soultana Tsitsikotas, Lily Ramyar, Aaron Pollett, Kara Semotiuk, Bharati Bapat The Journal of Molecular Diagnostics Volume 12, Issue 6, Pages 757-764 (November 2010) DOI: 10.2353/jmoldx.2010.090240 Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Representative chromatograms for cases 5A and 6. The left panels (A and C) indicate genomic DNA (gDNA) and the right panels (B and D) indicate complementary DNA (cDNA). Case 5A demonstrates decreased levels of the variant (GC) allele relative to the wild-type (AA) allele, while case 6 shows no change in the levels of the variant (C) allele relative to the wild-type (T) allele. Arrows highlight the heterozygous peaks. Both chromatograms shown were generated using the respective forward primer. (Blue: C, Green: A, Red: T, Black: G). The Journal of Molecular Diagnostics 2010 12, 757-764DOI: (10.2353/jmoldx.2010.090240) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions