Field community comparisons via 16S and between sequencing runs.

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Field community comparisons via 16S and between sequencing runs. Field community comparisons via 16S and between sequencing runs. (a) Good replication of rank abundance curves between different sequencing runs and with slightly different primers (515F-C is the original EMP primer and 515F-Y is the version where a C is replaced with a Y [16]). The abundance rank was defined by primer 515F-C. (b) Comparison of rank abundance curves from the June 2015 sample analysis, showing the aberrant sequencing run and the exact same PCR products reanalyzed on the other sequencing run on a different day. The abundance rank was defined by the rerun. Yi-Chun Yeh et al. mSystems 2018; doi:10.1128/mSystems.00023-18