Inhibition of β-catenin by the Smad4/BMP pathway.

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Inhibition of β-catenin by the Smad4/BMP pathway. Inhibition of β-catenin by the Smad4/BMP pathway. A and B, Ctnnb1 mRNA expression in primary cultured cells according to Smad4 status. A, real-time RT-PCR for Ctnnb1 mRNA in primary cultured gastric adenocarcinomas cells from Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+ and Pdx-1-Cre;Trp53F/F;Cdh1F/F mice. Expression levels of Ctnnb1 after treatment with 100 ng/mL of BMP2, 400 ng/mL of noggin (a BMP antagonist), and 5 ng/mL of TGFβ1 for 16 hours. B, i, real-time RT-PCR for Ctnnb1 mRNA in Smad4-overexpressing stable transfectants of Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+ primary cells (Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+-Smad4) versus vector-only transfected Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+ primary cells (Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+-Mock). ii, Smad4 Western blot analyses on B, i. C and D, β-catenin reporter activity in A and B. Tcf/Lef reporter activity was lower in Smad4-expressing cell lines (Pdx-1-Cre;Trp53F/F;Cdh1F/F and Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+-Smad4) than in Smad4-null cells. E, migratory activity of primary cultured cells from gastric adenocarcinomas arising from Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+ (n = 4) and Pdx-1-Cre;Trp53F/F;Cdh1F/F mice (n = 2). F, migratory activity of two independent primary cultured cell lines from gastric adenocarcinomas arising from Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+ mice, after knockdown of β-catenin using two different short hairpin RNAs. Western blot analysis showed the efficiency of β-catenin knockdown. G, representative image for shCtnnb1-1–tranfected Pdx-1-Cre;Smad4F/F;Trp53F/F;Cdh1F/+ cell line 2 (spindle-like shape) is shown with scrambled control-transfected cells (epithelial, cuboidal shape). H, β-catenin knockdown-induced changes in mRNA expression of Cdh2, MMP7, and EMT-activating transcription factors in G. *, P < 0.05. Bars, SEM. Representative data for repeated experiments are shown. Jun Won Park et al. Mol Cancer Res 2014;12:1088-1099 ©2014 by American Association for Cancer Research