Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Slides:

Advertisements

Similar presentations

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet Bharani Srinivasan,

Advertisements

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.) Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification of common allergenic structures in mugwort and ragweed pollen Reinhold Hirschwehr, MDa, Claudia Heppner, MSca, Susanne Spitzauer, MDb,

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Frequent occurrence of T cell–mediated late reactions revealed by atopy patch testing with hypoallergenic rBet v 1 fragments Raffaela Campana, PhD, Katharina.

Development and characterization of a recombinant, hypoallergenic, peptide-based vaccine for grass pollen allergy Margarete Focke-Tejkl, PhD, Milena.

Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy Raphaela.

Skin test diagnosis of grass pollen allergy with a recombinant hybrid molecule Carine Metz-Favre, MD, Birgit Linhart, PhD, Margarete Focke-Tejkl, PhD,

Peanut oleosins associated with severe peanut allergy—importance of lipophilic allergens for comprehensive allergy diagnostics Christian Schwager, Dipl.

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Correlation of IgE/IgG4 milk epitopes and affinity of milk-specific IgE antibodies with different phenotypes of clinical milk allergy Julie Wang, MD,

Usefulness of recombinant γ-gliadin 1 for identifying patients with celiac disease and monitoring adherence to a gluten-free diet Bharani Srinivasan,

Molecular cloning, expression, and characterization of a major 38-kd cochineal allergen Yoko Ohgiya, MS, Fumihiro Arakawa, MS, Hiroshi Akiyama, PhD, Yasuo.

Sabine Fischer, MSc,a, Monika Grote, PhD,b, B. Fahlbusch, PhD,c, W. D

Birch pollen immunotherapy results in long-term loss of Bet v 1–specific TH2 responses, transient TR1 activation, and synthesis of IgE-blocking antibodies

Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil Cristiana Alonzi, MD, Paolo Campi, MD, Francesco.

Antibody conjugates bispecific for intercellular adhesion molecule 1 and allergen prevent migration of allergens through respiratory epithelial cell layers

Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines

In vivo allergenic activity of a hypoallergenic mutant of the major fish allergen Cyp c 1 evaluated by means of skin testing Nikolaos Douladiris, MD,

Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch pollen allergens: Different allergenicity of Bet v 1 and Bet v 2 Susanne.

Molecular determinants of allergen-induced effector cell degranulation

Staphylococcus aureus fibronectin-binding protein specifically binds IgE from patients with atopic dermatitis and requires antigen presentation for cellular.

Anaphylactic reaction to (mixed) fruit juice containing dragon fruit

Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing Susanne Heiss, Rudolf Valenta

Allergen microarray detects high prevalence of asymptomatic IgE sensitizations to tropical pollen-derived carbohydrates Clarissa R. Cabauatan, PhD, Christian.

Major cat and dog allergens share IgE epitopes

Dissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5 Margarete Focke-Tejkl, PhD, Raffaela Campana, PhD, Renate.

Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population Susanne Krause, PhD, Gerald Reese, PhD, Stefanie.

Protein unfolding strongly modulates the allergenicity and immunogenicity of Pru p 3, the major peach allergen Masako Toda, PhD, Gerald Reese, PhD, Gabriele.

Characterization of a Novel Isoform of α-Nascent Polypeptide-associated Complex as IgE-defined Autoantigen Roschanak Mossabeb, Susanne Seiberler, Irene.

Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis Srinita Banerjee, Yvonne Resch, Kuan-Wei Chen, Ines.

Genetically engineered hybrid proteins from Parietaria judaica pollen for allergen- specific immunotherapy Roberto González-Rioja, PhD, Ignacio Ibarrola,

A hypoallergenic variant of Der p 1 as a candidate for mite allergy vaccines David Walgraffe, PhD, Christel Mattéotti, PhD, Mohamed el Bakkoury, PhD,

Kerstin Bauermeister, MSc, Barbara K

Underestimation of house dust mite–specific IgE with extract-based ImmunoCAPs compared with molecular ImmunoCAPs Huey-Jy Huang, MSc, Yvonne Resch-Marat,

IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1 Christiane Hilger, PhD,

Melanie Albrecht, MSc, Yvonne Kühne, MSc, Barbara K

Selection of house dust mite–allergic patients by molecular diagnosis may enhance success of specific immunotherapy Kuan-Wei Chen, PhD, Petra Zieglmayer,

Three-dimensional structure and IgE-binding properties of mature fully active Der p 1, a clinically relevant major allergen Sonia de Halleux, MSc, Enrico.

Spatial clustering of the IgE epitopes on the major timothy grass pollen allergen Phl p 1: Importance for allergenic activity Sabine Flicker, PhD, Peter.

Different IgE recognition of mite allergen components in asthmatic and nonasthmatic children Yvonne Resch, MSc, Sven Michel, MSc, Michael Kabesch, MD,

Quantitative IgE inhibition experiments with purified recombinant allergens indicate pollen-derived allergens as the sensitizing agents responsible for.

Skin test evaluation of a novel peptide carrier–based vaccine, BM32, in grass pollen– allergic patients Verena Niederberger, MD, Katharina Marth, MD,

Reshaping the Bet v 1 fold modulates TH polarization

Association between specific timothy grass antigens and changes in TH1- and TH2-cell responses following specific immunotherapy Véronique Schulten, PhD,

Identification of a polygalacturonase as a major allergen (Pla a 2) from Platanus acerifolia pollen Ignacio Ibarrola, PhD, M. Carmen Arilla, PhD, Alberto.

Soybean (Glycine max) allergy in Europe: Gly m 5 (β-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions.

A hypoallergenic cat vaccine based on Fel d 1–derived peptides fused to hepatitis B PreS Katarzyna Niespodziana, MSc, Margarete Focke-Tejkl, PhD, Birgit.

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

α-Purothionin, a new wheat allergen associated with severe allergy

Lipid transfer protein (Ara h 9) as a new peanut allergen relevant for a Mediterranean allergic population Susanne Krause, PhD, Gerald Reese, PhD, Stefanie.

Recombinant allergen-based IgE testing to distinguish bee and wasp allergy Irene Mittermann, PhD, Mihaela Zidarn, MD, Mira Silar, PhD, Zora Markovic-Housley,

Fungal protein MGL_1304 in sweat is an allergen for atopic dermatitis patients Takaaki Hiragun, MD, PhD, Kaori Ishii, MSc, Makiko Hiragun, MD, Hidenori.

Cat IgA, representative of new carbohydrate cross-reactive allergens

Hypoallergenic derivatives of the major birch pollen allergen Bet v 1 obtained by rational sequence reassembly Raffaela Campana, PhD, Susanne Vrtala,

Regina Selb, PhD, Julia Eckl-Dorna, MD, PhD, Teresa E

Allergy to human seminal fluid: Cross-reactivity with dog dander

Cloning, sequencing, and recombinant production of Sin a 2, an allergenic 11S globulin from yellow mustard seeds Oscar Palomares, PhD, Andrea Vereda,

Adverse reaction to lupine-fortified pasta

The IgE-Reactive Autoantigen Hom s 2 Induces Damage of Respiratory Epithelial Cells and Keratinocytes via Induction of IFN-γ Irene Mittermann, Renate.

Identification of wheat gliadins as an allergen family related to baker's asthma Cordula Bittner, MD, Britta Grassau, MS, Karsten Frenzel, PhD, Xaver.

Gastrointestinal digestion of Bet v 1–homologous food allergens destroys their mediator- releasing, but not T cell–activating, capacity Eva Maria Schimek,

Placental transfer of allergen-specific IgG but not IgE from a specific immunotherapy– treated mother Sabine Flicker, PhD, Katharina Marth, MD, Heinz.

Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy Teresa E. Twaroch, MSc, Margit Focke, PhD, Vera Civaj, Milena.

Anna Gieras, PhD, Birgit Linhart, PhD, Kenneth H

Molecular cloning of a major Alternaria alternata allergen, rAlt a 2

Presentation transcript:

Hypoallergenic Der p 1/Der p 2 combination vaccines for immunotherapy of house dust mite allergy Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R. Thomas, PhD, Ines Swoboda, PhD, Peter Valent, MD, Rudolf Valenta, MD, Susanne Vrtala, PhD Journal of Allergy and Clinical Immunology Volume 130, Issue 2, Pages 435-443.e4 (August 2012) DOI: 10.1016/j.jaci.2012.05.035 Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Construction of Der p 2/Der p 1 mosaic proteins. Three fragments of Der p 1 (1.1, 1.2, and 1.3) comprising amino acids 1 to 84, 85 to 143, and 144 to 222 and 2 fragments of Der p 2 (2.1 and 2.2) comprising amino acids 1 to 53 and 54 to 129 were reassembled in the order 1.3, 2.2, 1.2, 2.1, and 1.1. Der p 2/1C contains the original amino acid sequence of Der p 1 and Der p 2, including the 12 cysteine residues (dashed vertical lines), whereas in Der p 2/1S the cysteine residues were exchanged for serine residues. Both proteins contain a hexahistidine tag at the C-terminus. Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Expression and purification of rDer p 2/Der p 1 mosaic proteins. Coomassie-stained SDS-PAGE–containing protein extracts of BL21 (DE3) expressing Der p 2/1C and Der p 2/1S (lanes 1), purified Der p 2/1C and Der p 2/1S (lanes 2; A), and purified Der p 2/1C and Der p 2/1S separated under reducing (lanes r) and nonreducing (lanes nr) conditions (B) and molecular weight markers (lanes M) are shown. Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Far-UV CD spectra of nDer p 1, rDer p 2, rDer p 2/1C, and rDer p 2/1S. The mean residue ellipticity (y-axis) at given wavelengths (x-axis) is shown for each protein. Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 IgE reactivity of nDer p 1, rDer p 2, and rDer p 2/1 mosaic proteins. Dot-blotted nDer p 1, rDer p 2, the 2 Der p 2/1 mosaic proteins, and BSA were tested for IgE reactivity with sera from 32 patients with HDM allergy (lanes 1-32), serum from a nonallergic subject (NC), or buffer without serum (BC). Bound IgE was detected with iodine 125–labeled anti-human IgE antibodies and visualized by using autoradiography. Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Allergenic activity of nDer p 1, rDer p 2, and rDer p 2/1 mosaic proteins. Basophils from 10 patients with HDM allergy were stimulated with various concentrations of nDer p 1, rDer p 2, rDer p 2/1C, and rDer p2/1S (x-axes). Expression of CD203c was determined by using fluorescence-activated cell-sorting analysis, and stimulation indices (SI) are shown (y-axes). Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Reactivity and epitope specificity of IgG antibodies induced by immunization of rabbits with nDer p 1, rDer p 2, and rDer p2/1 mosaic proteins. Sera obtained from rabbits before (pre) or after (immune) immunization with nDer p 1, rDer p 2, rDer p 2/1C, and rDer p 2/1S were tested for IgG reactivity to dot-blotted nDer p 1, rDer p 2, rDer p 2/1 mosaic proteins, and BSA or with synthetic Der p 1– and Der p 2–derived peptides (Der p 1 P1-P8 and Der p 2 P1-P5). Bound IgG antibodies were detected with iodine 125–labeled donkey anti-rabbit IgG antibodies. Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 7 Reduced allergenicity of the 2 rDer p 2/1 mosaic proteins. RBL cells were loaded with sera from mice obtained before (preimmune) or after (immune) immunization with nDer p 1, rDer p 2, or the 2 mosaic proteins (x-axes). nDer p 1–specific (A) and rDer p 2–specific (B) release of β-hexosaminidase are displayed as percentages of total β-hexosaminidase release (medians ± SDs for the 5 sera from each group of mice, y-axes). Journal of Allergy and Clinical Immunology 2012 130, 435-443.e4DOI: (10.1016/j.jaci.2012.05.035) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions