Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid M.P. Brown, D.V.M., M.Sc., T.N.

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Presentation transcript:

Exercise and injury increase chondroitin sulfate chain length and decrease hyaluronan chain length in synovial fluid M.P. Brown, D.V.M., M.Sc., T.N. Trumble, D.V.M., Ph.D., A.H.K. Plaas, Ph.D., J.D. Sandy, Ph.D., M. Romano, B.S., J. Hernandez, D.V.M., Ph.D., K.A. Merritt, B.S. Osteoarthritis and Cartilage Volume 15, Issue 11, Pages 1318-1325 (November 2007) DOI: 10.1016/j.joca.2007.04.005 Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Plot of mean SF and cartilage CS concentrations (μg/ml of SF or μg/mg of cartilage; +SD) vs Superose 6 fraction number. Chain length progressively decreases from fraction B11 to B24. (A)–(C) CS in SF from horses with normal joints after rest and exercise (n=9 each; same joints compared) and from horses undergoing arthroscopic surgery for OC fragment removal (n=11). Note that CS chain length in SF from OC injured joints is longer than that seen in the rested and exercised groups. (D) CS in cartilage from the same horses undergoing arthroscopic surgery for OC fragment removal (n=9). Note that CS chain length in cartilage from OC joints is similar to that seen in the SF from these joints. Differences between groups (P<0.05): + – OC injury vs rested; * – OC injury vs rested and exercised; # – OC injury vs exercised. Osteoarthritis and Cartilage 2007 15, 1318-1325DOI: (10.1016/j.joca.2007.04.005) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Horizontal scatter plot of peak SF CS Superose 6 fractions for each joint in the OC injury, rested, and exercised groups. The vertical dashed lines represent the dividing line between fractions B18 (18.7kDa) and B19 (15.6kDa). The short vertical solid lines represent the mean value for each group. Osteoarthritis and Cartilage 2007 15, 1318-1325DOI: (10.1016/j.joca.2007.04.005) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Plot of mean serum CS concentrations (μg/ml+SD) vs Superose 6 fraction number. (A) Δdi0S in serum from horses with normal joints after rest and exercise (collected immediately after and then 1h after stress test; n=9) and from horses undergoing arthroscopic surgery for OC fragment removal (n=9). (B) Δdi4S in serum from same horses. Note the peaks in OC injury serum occur in fraction B18 (18.7kDa), similar to those seen in SF and cartilage of this group (see Fig. 1). Differences between groups (P<0.05): + – OC injury vs rested; # – OC injury vs exercised. Osteoarthritis and Cartilage 2007 15, 1318-1325DOI: (10.1016/j.joca.2007.04.005) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Plot of mean SF HA concentrations (μg/ml+SD) vs Sephacryl S-1000 fraction (A7–A24) or Superose 6 fraction(B7–B23) from horses with normal joints after rest and exercise (n=9 each; same joints compared) and from horses undergoing arthroscopic surgery for OC fragment removal (n=11). Differences between groups (P<0.05): + – OC injury vs rested; * – OC injury vs rested and exercised; # – OC injury vs exercised; ▿ – rested vs OC injury and exercised; ∞ rested vs exercised. Total HA concentration (μg/ml; AUC) for the OC injury group was significantly lower than for the rested group (P<0.05). Osteoarthritis and Cartilage 2007 15, 1318-1325DOI: (10.1016/j.joca.2007.04.005) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Plot of mean cartilage HA concentrations (μg/mg+SD) vs Superose 6 fraction (B7–B23) from horses with OC injury (n=9). Mean total HA content in these samples was 0.18±0.16μg/mg (AUC±SD) of cartilage, with the peak occurring at B10. Osteoarthritis and Cartilage 2007 15, 1318-1325DOI: (10.1016/j.joca.2007.04.005) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions