Whole-genome comparison of E

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Whole-genome comparison of E Whole-genome comparison of E. coli TolC to closely related strains and a schematic presentation of transposon-mediated disruption of the tolC CDS in E. coli TolC. Whole-genome comparison of E. coli TolC to closely related strains and a schematic presentation of transposon-mediated disruption of the tolC CDS in E. coli TolC. (A) BLAST ring image generator (BRIG) (64) comparison of the E. coli TolC genome (innermost black ring) to the closely related genomes of E. coli strains K-12 MG1655, ER1821R, NCM3722, K-12 W3110, and JW5437-1 (the four outermost rings), shown in blue to red, respectively, as identified by isDDH (29). Shading of the four outermost rings is according to their respective percent nucleotide identity to the query sequence (E. coli TolC), determined by BLAST+. The second and third innermost rings show the GC skew (purple/green) and the GC content (black). IS5 elements are numbered according to annotations for E. coli K-12 MG1655 (NC_000913.3). The location of the fertility plasmid on the chromosome of E. coli TolC is indicated by the letter F (on left side of diagram). (B) Close-up comparison of the tolC locus of E. coli TolC and its closest relative, E. coli K-12 MG1655, drawn by using Easyfig (66) The tolC locus (tolC_1 and tolC_2) in E. coli TolC is interrupted by insertion of an IS5 element (IS5*) that codes for the transposase insH1 (ins5A). ins5B (**) and ins5C (*) are indicated by arrows in reverse orientation, underneath insH1. A BLAST+ comparison of the tolC locus for each of the two strains indicated 100% nucleotide identity. Jannik Donner et al. mSphere 2017; doi:10.1128/mSphereDirect.00375-17