In vitro protection of vascular function from oxidative stress and inflammation by pulsatility in resistance arteries Frédéric Pinaud, MD, Laurent Loufrani,

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Presentation transcript:

In vitro protection of vascular function from oxidative stress and inflammation by pulsatility in resistance arteries Frédéric Pinaud, MD, Laurent Loufrani, PhD, Bertrand Toutain, MS, Diane Lambert, PhD, Lionel Vandekerckhove, BSc, Daniel Henrion, PharmD, PhD, Christophe Baufreton, MD, PhD, FETCS The Journal of Thoracic and Cardiovascular Surgery Volume 142, Issue 5, Pages 1254-1262 (November 2011) DOI: 10.1016/j.jtcvs.2011.07.007 Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Typical recording showing blood pressure measurements obtained in vivo in the carotid artery (A) and those obtained in a mesenteric resistance artery perfused in vitro in an arteriograph and subjected to pulsatility (B). When pulsatility was started (arrow, C), pulsatility was detectable both on the diameter recording (C) and on the pressure recording (D). Corresponding control recordings (artery without pulsatility) are shown for diameter (E) and pressure (F). The lower panel (G) shows diameter changes in response to pulsatility (red trace) relative to nonpulsatile arteries. Pulsatility was maintained for 180 minutes. Data are presented as mean ± SEM (n = 10 rats per group). The Journal of Thoracic and Cardiovascular Surgery 2011 142, 1254-1262DOI: (10.1016/j.jtcvs.2011.07.007) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Flow-mediated dilation (A) and myogenic tone (B) in mesenteric resistance arteries subjected to pulsatile (P) or nonpulsatile (NP) conditions for 30 (left panel) or 180 (right panel) minutes. Data are presented as mean ± SEM (n = 10 rats per group). Asterisk indicates P < .01 for pulsatile versus nonpulsatile conditions. The Journal of Thoracic and Cardiovascular Surgery 2011 142, 1254-1262DOI: (10.1016/j.jtcvs.2011.07.007) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Reactive oxygen species (ROS) detection with dihydroethydine (DHE). Reactive oxygen species level was determined in mesenteric resistance arteries subjected to pulsatile (P) or nonpulsatile (NP) conditions for 0, 30, or 180 minutes. Data are presented as mean ± SEM (n = 10 rats per group). Asterisk indicates P < .01 for pulsatile versus nonpulsatile conditions. The Journal of Thoracic and Cardiovascular Surgery 2011 142, 1254-1262DOI: (10.1016/j.jtcvs.2011.07.007) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 Monocyte chemotactic protein 1 (MCP-1, A) and tumor necrosis factor α (TNFα, B) levels were determined with immunohistochemical methods (IHC) in mesenteric resistance arteries subjected to pulsatile or nonpulsatile conditions for 30 or 180 minutes. Typical images are shown on the left side. Positive (Pos.) and negative (Neg.) controls are shown for each protein. Tumor necrosis factor α level was then measured in the perfusate of whole mesenteric arterial beds perfused under pulsatile or nonpulsatile conditions (C). Perfusate was collected after 30, 90, and 180 minutes. Data are presented as mean ± SEM (n = 10 rats per group). Asterisk indicates P < .01 for pulsatile versus nonpulsatile conditions. The Journal of Thoracic and Cardiovascular Surgery 2011 142, 1254-1262DOI: (10.1016/j.jtcvs.2011.07.007) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 Effect of treatment with the antioxidant tempol on pulsatility-induced dilation (A), on flow-mediated dilation (B and D), and on myogenic tone (C and E). Nuclear factor κB (NFκB, F) and monocyte chemotactic protein 1 (MCP-1, G) protein levels were measured in arterial walls subjected to pulsatile (P) or nonpulsatile (NP) conditions in the presence or absence of tempol. Flow-mediated dilation and myogenic tone were determined after 30 (B and C) or 180 (D and E) minutes. Protein level was determined with Western blot analysis. Data are presented as mean ± SEM (n = 10 rats per group). Asterisk indicates P < .01 for pulsatile versus nonpulsatile conditions. Hatch mark indicates P < .01 for the effect of tempol. The Journal of Thoracic and Cardiovascular Surgery 2011 142, 1254-1262DOI: (10.1016/j.jtcvs.2011.07.007) Copyright © 2011 The American Association for Thoracic Surgery Terms and Conditions