Sperm head's birefringence: a new criterion for sperm selection

Slides:

Advertisements

Similar presentations

Laura Londra, M. D. , Caroline Moreau, M. D. , M. P. H. , Ph. D

Advertisements

Impact of oocyte cryopreservation on embryo development

Report of results obtained in 2,934 women using donor sperm: donor insemination versus in vitro fertilization according to indication Thamara Viloria,

Effect of chronic hypertension on assisted pregnancy outcomes: a population-based study in Ontario, Canada Natalie Dayan, M.D., Andrea Lanes, M.Sc.,

Successful pregnancy and delivery of two sets of monozygotic twins after intracytoplasmic sperm injection and embryo transfer: case report and literature.

The relative effects of hormones and relationship factors on sexual function of women through the natural menopausal transition Lorraine Dennerstein,

Comparison of Sperm Motility Measurement Using SQA-V Automated Sperm Analyzer and Conventional Manual Methods R.K. Sharma, T.M. Said, A. Agarwal Fertility.

Fernando Zegers-Hochschild, M.D. Fertility and Sterility

Triggering with human chorionic gonadotropin or a gonadotropin-releasing hormone agonist in gonadotropin-releasing hormone antagonist-treated oocyte donor.

Michael M. Alper, M.D. Fertility and Sterility

Elena Moretti, Ph. D. , Giulia Collodel, Ph. D

Zhi-Yong Yang, M.D., Ri-Cheng Chian, Ph.D. Fertility and Sterility

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Robot-assisted laparoscopy for infertility treatment: current views

Can an educational DVD improve the acceptability of elective single embryo transfer? A randomized controlled study Nicole Hope, M.B., B.S., Hon., Luk.

Isabel Cerveira, M. D. , Cristina Costa, M. D. , Fernando Santos, M. D

Comparison of fallopian tube sperm perfusion and intrauterine tuboperitoneal insemination: a prospective randomized study Leonidas Mamas, M.D., Ph.D.

Azoospermia: is sample centrifugation indicated

Medical treatment of ectopic pregnancy: a committee opinion

Preimplantation genetic testing: polar bodies, blastomeres, trophectoderm cells, or blastocoelic fluid? M. Cristina Magli, M.Sc., Alessandra Pomante,

Live birth of twins derived from zona-free oocytes

How old is too old? Challenges faced by clinicians concerning age cutoffs for patients undergoing in vitro fertilization Robert L. Klitzman, M.D. Fertility.

Primary hyperparathyroidism in pregnancy

Oocyte cryopreservation

Aneuploidies of chromosomes 1, 4, and 6 are not compatible with human embryos' implantation M. Cristina Magli, M.Sc., Luca Gianaroli, M.D., Andor Crippa,

Looking back: egg donors' retrospective evaluations of their motivations, expectations, and experiences during their first donation cycle Nancy J. Kenney,

Influence of acupuncture stimulation on pregnancy rates for women undergoing embryo transfer Caroline Smith, Ph.D., Meaghan Coyle, B.Hlth.Sc. (Acup.),

A comparison of three downregulation approaches for poor responders undergoing in vitro fertilization Laura Detti, M.D., Daniel B. Williams, M.D., Jared.

A meta-analysis of the route of administration of luteal phase support in assisted reproductive technology: vaginal versus intramuscular progesterone

Alternate indications for varicocele repair: non-obstructive azoospermia, pain, androgen deficiency and progressive testicular dysfunction Peter N. Schlegel,

Expression of two-pore domain potassium channels in nonhuman primate sperm Gregory E. Chow, M.D., Charles H. Muller, Ph.D., Eliza C. Curnow, M.Sc., Eric.

DNA integrity is maintained after freeze-drying of human spermatozoa

Programming the endometrium for deferred transfer of cryopreserved embryos: hormone replacement versus modified natural cycles Eva R. Groenewoud, M.D.,

Management of tubal ectopic pregnancy: methotrexate and salpingostomy are preferred to preserve fertility Stephanie Beall, M.D., Ph.D., Alan H. DeCherney,

Polarization microscopy imaging for the identification of unfertilized oocytes after short- term insemination Yi Guo, M.D., Wenqiang Liu, Ph.D., Yu Wang,

Venous thromboembolism in relation to in vitro fertilization: an approach to determining the incidence and increase in risk in successful cycles Karin.

Should embryos developing to blastocysts on day 7 be cryopreserved and transferred: an analysis of pregnancy and implantation rates George Kovalevsky,

Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis Aylin Pelin Cil, M.D., Heejung Bang, Ph.D.,

What is the best frame rate for evaluation of sperm motility in different species by computer-assisted sperm analysis? Cesare Castellini, Ph.D., Alessandro.

Ongoing twin pregnancy after rescue intracytoplasmic sperm injection of unfertilized abnormal oocytes Navid Esfandiari, D.V.M., Ph.D., H.C.L.D., E. Anne.

Akanksha Mehta, M.D., Darius A. Paduch, M.D., Ph.D.

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Number of good quality embryos on day 3 is predictive for both pregnancy and implantation rates in in vitro fertilization/intracytoplasmic sperm injection.

Poor sperm quality affects clinical outcomes of intracytoplasmic sperm injection in fresh and subsequent frozen–thawed cycles: potential paternal effects.

“Information-rich” reproductive outcomes in carriers of a structural chromosome rearrangement ascertained on the basis of recurrent pregnancy loss Michelle.

Douglas T. Carrell, Ph.D., Benjamin R. Emery, B.S.

Zsolt P. Nagy, M. D. , Ph. D. , Laura F. Rienzi, Ph. D. , Filippo M

Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D

Website quality assessment: Mistaking apples for oranges

Recai Pabuccu, M. D. , Gogsen Onalan, M. D. , Umit Goktolga, M. D

Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.

Spindle imaging: a marker for embryo development and implantation

Fertility and Sterility: an evaluation

Physical health at 5.5 years of age of term-born singletons after intracytoplasmic sperm injection: results of a prospective, controlled, single-blinded.

Failed IVF cycles and the risk of subsequent preeclampsia or fetal growth restriction: a case-control exploratory study Ioanna Tsoumpou, M.D., Ahmed.

Cognitive development of singletons born after intracytoplasmic sperm injection compared with in vitro fertilization and natural conception Marjolein.

Impact of subclinical hypothyroidism in women with recurrent early pregnancy loss Lia A. Bernardi, M.D., Ronald N. Cohen, M.D., Mary D. Stephenson, M.D.,

Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome Mehdi Benchaib, M.D., Ph.D., Jacqueline.

Genetic evaluation procedures at sperm banks in the United States

In vitro sildenafil citrate use as a sperm motility stimulant

Cumulative live birth rates in cohorts of patients treated with in vitro fertilization or intracytoplasmic sperm injection Cynthia Witsenburg, M.D.,

Birefringence characteristics in sperm heads allow for the selection of reacted spermatozoa for intracytoplasmic sperm injection Luca Gianaroli, M.D.,

Evidence of absence or absence of evidence

Contact among families who share the same sperm donor

Effect of oocyte quality on blastocyst development after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in a sheep model Maria.

Myung-Geol Pang, Ph. D. , Young-Ah You, M. S. , Yoo-Jin Park, M. S

Is chromosome testing of the second miscarriage cost saving

Numerical chromosome anomalies detected in paternally derived pronuclei of tripronuclear zygotes after intracytoplasmic sperm injection Ervin Macas,

In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection Sílvia Mateo,

Male age negatively impacts embryo development and reproductive outcome in donor oocyte assisted reproductive technology cycles John L. Frattarelli,

Presentation transcript:

Sperm head's birefringence: a new criterion for sperm selection Luca Gianaroli, M.D., M. Cristina Magli, M.Sc., Giulia Collodel, Ph.D., Elena Moretti, Ph.D., Anna P. Ferraretti, M.D., Baccio Baccetti, M.D. Fertility and Sterility Volume 90, Issue 1, Pages 104-112 (July 2008) DOI: 10.1016/j.fertnstert.2007.05.078 Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 1 The acrosome is reacted in spermatozoon (A) and not reacted in sperm (B). Sperm C is devoid of birefringence because of the absence of a conventional sperm texture, with a vacuole in its head. Only nonpyknotic nuclei can be birefringent, because of the organization of nucleoprotein filaments, strictly packed in a very compact texture. Fertility and Sterility 2008 90, 104-112DOI: (10.1016/j.fertnstert.2007.05.078) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 2 The ICSI procedure is performed under the polarizing light. A birefringent spermatozoon is selected and immobilized (A), aspirated (B), injected (C), and released in the ooplasm (D). The birefringence of the head is evident at every step of ICSI. Fertility and Sterility 2008 90, 104-112DOI: (10.1016/j.fertnstert.2007.05.078) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 3 The polarization inverted microscope equipped with the micromanipulators seen in frontal view. The light source, the polarizer, the compensator, and the analyzer are indicated. There are two cameras, one transmitting the image to the computer and the other to the monitor. Fertility and Sterility 2008 90, 104-112DOI: (10.1016/j.fertnstert.2007.05.078) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Pregnancy rate per ET (A) and ongoing pregnancy rate (B) in relation to the quality of the sperm sample. Bars with same letters are statistically significantly different. OAT = oligoasthenoteratospermic. Fertility and Sterility 2008 90, 104-112DOI: (10.1016/j.fertnstert.2007.05.078) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Ongoing pregnancy rate per cycle (A) and implantation rate (B) in relation to the quality of the sperm sample. Bars with same letters are statistically significantly different. OAT = oligoasthenoteratospermic. Fertility and Sterility 2008 90, 104-112DOI: (10.1016/j.fertnstert.2007.05.078) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions