S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

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Application of polymerase chain reaction for detection of Legionella pneumophila in serum samples S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A.F. Lambropoulos, A. Antoniadis Clinical Microbiology and Infection Volume 4, Issue 3, Pages 144-148 (March 1998) DOI: 10.1111/j.1469-0691.1998.tb00377.x Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Gel electrophoresis of PCR products. L: DNA we markers with the 123-bp band shown. (A) Lane 1, PCR product of positive control DNA. Lanes 3, 7, 8, 9, 11, 16 and 17 show the PCR products of patient sera. (B) Lane 1, PCR product of negative control. Lanes 3. 4, 6, 7 and 8 show the PCR products of patient sera. Clinical Microbiology and Infection 1998 4, 144-148DOI: (10.1111/j.1469-0691.1998.tb00377.x) Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Southern blot hybridization of the amplified products with the internal oligonucleotide probe. The sequence of the probe is described in the text. (A) Lane 1, positive control. Amplified products in lanes 3, 8, 9, 11, 16 and 17 hybridized with the L5S-l probe. (B) Lane 1, negative control. PCR products in lanes 2, 3, 4, 7, 8 and 11 hybridized with the same probe. Clinical Microbiology and Infection 1998 4, 144-148DOI: (10.1111/j.1469-0691.1998.tb00377.x) Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions