PATHFAST® Training Belo Horizonte, June 16, 2011

Characteristics Compact immunoanalyzer Full Automation Whole blood, plasma, serum Multi item processing ( up to six) Short Assay Time ( 17 minutes) Easy to Use Hight Sensitivity / Acccuracy/ Reproducibility Wide Dinamic Range Minimun Cross Contamination Barcode Control Sample Recognition sensor

PATHFAST® Assay Protocol Whole Blood / Plasma Analyte Contaminant Immunoreaction 5 min Ｙ Y A L P Ｙ Y A L P ALP Conjugate Magnetic Particles Ｙ Y A L P 4 7 n m P M T C a l c u t o r BF washing Chemilumi Substrate Enzyme Reaction 1min Measure Light Emission CDP-Star

Kits Each kit contains; Reagent cartridges (6 cartridges x 10 boxes) Calibrators (2 levels x 2 bottles) Calibrator Diluents ( 4 tubes) …for cTnI, CK-MB, D-Dimer Package Insert (IFU) & MC Entry Card *Stored at 2 to 8 ℃

Reagent Cartridge ■Magnetic particles ■ALP conjugate ■Chemilumin. Substrate (CDP-Star with SapphireII) ●Sample Diluent ●Washing Buffer ■ ● ■ ■ ● ● ● Sample Well Detection Well

Reagent Cartridge Assay item Lot number Lot expiration Detection well Sample well Cartridge Serial Number

Calibrators & Controls Calibrators & Calibrator Diluents are included. Controls are not included. MKI suggests users to use Biorad controls. PATHFAST data are described in package insert of Biorad controls.

PATHFAST®Disposables PATHFAST TIP PATHFAST Waste Box Change once a week (10 boxes / package) Printer Roll Paper Use one per test (42 tips x 5 boxes / Carton) (10 rolls / package)

4. PATHFAST® Components

PATHFAST® Critical Care Immunology Analyzer manufactured on the basis of an outstanding engineering experience and excellent and well known reagent quality of Mitsubishi.