Association of electrostimulation with cell transplantation in ischemic heart disease Abdel Shafy, MD, Thomas Lavergne, MD, Christian Latremouille, MD,

Slides:

Advertisements

Similar presentations

Total arterial revascularization of triple vessel coronary disease based on combined internal thoracic and radial artery grafts Robert H. Habib, PhD,

Advertisements

Manuel J. Antunes, MD, PhD, DSc

Aortic valve replacement in low-flow, low-gradient aortic stenosis: Left ventricular ejection fraction matters Victor Dayan, MD, PhD, Philippe Pibarot,

Therapeutic Effect of Midkine on Cardiac Remodeling in Infarcted Rat Hearts Shinya Fukui, MD, Satoru Kitagawa-Sakakida, MD, PhD, Sin Kawamata, MD, PhD,

Epicardial infarct repair with basic fibroblast growth factor–enhanced CorMatrix-ECM biomaterial attenuates postischemic cardiac remodeling Holly E.M.

A combination of omental flap and growth factor therapy induces arteriogenesis and increases myocardial perfusion in chronic myocardial ischemia: Evolving.

Myocardial bridging over the left anterior descending: Myotomy, bypass, or both? Chigozirim N. Ekeke, BS, Stephen Noble, MD, Ernest Mazzaferri, MD, Juan.

Direct epicardial shock wave therapy improves ventricular function and induces angiogenesis in ischemic heart failure Daniel Zimpfer, MD, Seyedhossein.

Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac.

Intramyocardial bone marrow stem cell transplantation during coronary artery bypass surgery: A meta-analysis Peter Donndorf, MD, Guenther Kundt, PhD,

The American Association for Thoracic Surgery 2016 Ethics Forum: Cost-effectiveness and the ethics of left ventricular assist device therapy John W.C.

Changfa Guo, MD, Husnain Kh. Haider, PhD, Winston S. N

Combined transplantation of skeletal myoblasts and angiopoietic progenitor cells reduces infarct size and apoptosis and improves cardiac function in chronic.

Changfa Guo, MD, Husnain Kh

Cardiac pheochromocytoma

Pheochromocytoma-induced inverted Takotsubo cardiomyopathy: A case of patient resuscitation with extracorporeal life support Rachid Zegdi, MD PhD, Caroline.

Persistence of marrow stromal cells implanted into acutely infarcted myocardium: Observations in a xenotransplant model Derek J. MacDonald, MD, Jun Luo,

Right internal thoracic artery or saphenous vein grafting

Which is more important in the potential interference on cardioprotection by remote ischemic preconditioning: β-blockers or anesthetic choice? Yang Liu,

Myocardial regeneration for chronic heart failure: Not as easy as it sounds Richard A. Hopkins, MD The Journal of Thoracic and Cardiovascular Surgery

Intrinsic cardiac stem cells are essential for regeneration

Preventing cardiac remodeling: The combination of cell-based therapy and cardiac support therapy preserves left ventricular function in rodent model of.

Juan A. Crestanello, MD, Richard C. Daly, MD

Centers for Disease Control “increased-risk” organ donor: Not so risky? Francis D. Pagani, MD, PhD The Journal of Thoracic and Cardiovascular Surgery

Why surgery won the SYNTAX trial and why it matters

Major complications during negative pressure wound therapy in poststernotomy mediastinitis after cardiac surgery Rainer Petzina, MD, PhD, Malin Malmsjö,

Multilevel data analysis: What? Why? How?

Improvement of cardiac function in the failing rat heart after transfer of skeletal myoblasts engineered to overexpress placental growth factor Matthias.

Cardiac dysfunction induced by experimental myocardial infarction impairs the host defense response to bacterial infection in mice because of reduced.

Novel regenerative therapy using cell-sheet covered with omentum flap delivers a huge number of cells in a porcine myocardial infarction model Yasuhiro.

Acute left ventricular failure after bilateral lung transplantation for idiopathic pulmonary arterial hypertension Tom Verbelen, MD, Sophie Van Cromphaut,

Right ventricular outflow tract resection for treatment of refractory ventricular tachycardia in a 2-year-old child S. Kanaan, MD, K. Shamsuddin, MD,

Who needs blood? The flip side of blood conservation

Congestive heart failure: Treat the disease, not the symptom

Juan N. Pulido, MD The Journal of Thoracic and Cardiovascular Surgery

For everything there is a season

Coronary blood supply of the inferior wall of the right ventricle in hearts with Ebstein malformation: relevance to vertical plication Iki Adachi, MD,

A first start for lung transplantation?

Right ventricular failure after cardiac surgery: Why can't right ventricular assist device support fix the problem? Francis D. Pagani, MD, PhD The Journal.

Robotic minimally invasive cell transplantation for heart failure

Angiogenic pretreatment to enhance myocardial function after cellular cardiomyoplasty with skeletal myoblasts Mauricio A. Retuerto, BS, James T. Beckmann,

Cellular therapy reverses myocardial dysfunction

Coronary artery thromboembolism as a result of left ventricular sump aneurysm after congenital heart surgery Timothy B. Cotts, MD, Felix J. Rogers, DO,

Joseph A. Dearani, MD, Michael J. Ackerman, MD, PhD

Layered implantation of myoblast sheets attenuates adverse cardiac remodeling of the infarcted heart Naosumi Sekiya, MD, Goro Matsumiya, MD, PhD, Shigeru.

Restoration of left ventricular geometry and improvement of left ventricular function in a rodent model of chronic ischemic cardiomyopathy Jiashing Yu,

Chronic obstructive pulmonary disease severity influences outcomes after off-pump coronary artery bypass Benjamin Medalion, MD, Michael G. Katz, MD,

Luís Roberto Gerola, MD, PhD, Hyong C

Guidelines should bother us, not comfort us

Elimination of Ischemic Mitral Regurgitation Does Not Alter Long-Term Left Ventricular Remodeling in the Ovine Model Kanji Matsuzaki, MD, PhD, Masato.

Grafted skeletal myoblast sheets attenuate myocardial remodeling in pacing-induced canine heart failure model Hiroki Hata, MD, Goro Matsumiya, MD, PhD,

Chronic septal infarction confers right ventricular protection during mechanical left ventricular unloading James Mau, BSc, MB, BS, Stuart Menzie, MB,

Yinsu Zhu, MD, Lijun Tang, MD, PhD, Yi Xu, MD

Zhi Cui, MSc, Ren-Ke Li, MD, PhD

Ralph S. Mosca, MD The Journal of Thoracic and Cardiovascular Surgery

Preserving the V-engine shape of the left ventricle with Melody mitral valve replacement in small children Patrick O. Myers, MD The Journal of Thoracic.

The origins of open heart surgery at the University of Minnesota 1951 to 1956 Richard A. DeWall, MD The Journal of Thoracic and Cardiovascular Surgery

Myoblast-seeded biodegradable scaffolds to prevent post–myocardial infarction evolution toward heart failure Matthias Siepe, MD, Marie-Noëlle Giraud,

The Journal of Thoracic and Cardiovascular Surgery

Discussion The Journal of Thoracic and Cardiovascular Surgery

The future of cardiac surgery training: A survival guide

Optimized ventricular restraint therapy: Adjustable restraint is superior to standard restraint in an ovine model of ischemic cardiomyopathy Lawrence.

The continuing challenge of congenital heart disease in China

Transplantation of cryopreserved muscle cells in dilated cardiomyopathy: Effects on left ventricular geometry and function Nobuhisa Ohno, MD, Paul W.M.

Aneurysmal right coronary with fistula to the coronary sinus combined with severe stenosis of the left anterior descending artery: A snake on the heart

Toward a more rational approach in treating type B aortic dissection

Appropriate Use Criteria for coronary revascularization in patients with stable ischemic heart disease: What the surgeon needs to know Harold L. Lazar,

Optimizing cardiac cell therapy: From processing to delivery

Respect the aorta The Journal of Thoracic and Cardiovascular Surgery

Impact of preoperative left ventricular function and time from infarction on the long-term benefits after intramyocardial CD133+ bone marrow stem cell.

Presentation transcript:

Association of electrostimulation with cell transplantation in ischemic heart disease Abdel Shafy, MD, Thomas Lavergne, MD, Christian Latremouille, MD, PhD, Miguel Cortes-Morichetti, MD, Alain Carpentier, MD, PhD, Juan C. Chachques, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 138, Issue 4, Pages 994-1001 (October 2009) DOI: 10.1016/j.jtcvs.2009.02.025 Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Study design. MI, Myocardial infarction. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Explanted heart at 12 weeks of myocardial infarction, created by the ligation of the distal homonymous (equivalent to human left anterior descending) and second diagonal coronary arteries. View from the apex of the right ventricle (RV), the left ventricle (LV), and the myocardial infarction (MI). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 A, Skeletal myoblasts after 4 weeks of in vitro culture (phase contrast, magnification ×100). B, The red asterisk shows cells that appear with a typical myoblast phenotype. The black asterisks show cells proliferating toward a myotube-like shape (magnification ×400). C, The arrows show nuclei with different morphology, from central round to peripheral elongated corresponding to different stages of skeletal muscle differentiation (magnification ×1000). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 4 A, Echocardiographic studies. Left ventricular end-diastolic volume (LVEDV) evolution during follow-up (∗P < .01). B, Ejection fraction (EF) evolution during follow-up (∗P < .01). C, Left ventricular anterior wall thickness at diastolic phase evolution during follow-up (∗P < .01). CRT, Cardiac resynchronization therapy; LVAWd, anterior wall thickness at diastolic phase. The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 5 A, Histoimmunologic studies at 12 weeks in cell-treated group, showing isolated grafted myoblasts into the infarcted area. Stain with MY-32 antibody to skeletal myosin (original magnification, ×250). B, Histoimmunologic studies at 12 weeks in the cell + electrostimulation group, showing a mixture of multinucleated myotubes and fibrillar configuration. Stain with MY-32 antibody to skeletal myosin (original magnification, ×200). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 6 Cross and longitudinal section of multinucleated myotubes (marked with 4′,6-diamidino-2-phenylindole dye) developed in the myocardial infarcted area after treatment with skeletal myoblasts associated with chronic electrostimulation (original magnification, ×360). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions

Figure 7 Left, Myoblasts organized and assembled in myofibers after pacing. Stain with antibody to slow-twitch skeletal myosin (original magnification, ×300). Right, Fibrillar configuration of myoblasts marked with 4′,6-diamidino-2-phenylindole dye in the infarcted area, group 4 (original magnification, ×320). The Journal of Thoracic and Cardiovascular Surgery 2009 138, 994-1001DOI: (10.1016/j.jtcvs.2009.02.025) Copyright © 2009 The American Association for Thoracic Surgery Terms and Conditions