Volume 13, Issue 11, Pages (November 2006)

Slides:



Advertisements
Similar presentations
Crystal Structure of Transcription Factor MalT Domain III
Advertisements

Structure of the Rho Family GTP-Binding Protein Cdc42 in Complex with the Multifunctional Regulator RhoGDI  Gregory R. Hoffman, Nicolas Nassar, Richard.
Crystal Structure of Glycogen Synthase Kinase 3β
The 1.4 Å Crystal Structure of Kumamolysin
Mechanism and Substrate Recognition of Human Holo ACP Synthase
Volume 21, Issue 4, Pages (April 2014)
Volume 20, Issue 6, Pages (June 2013)
Arvin C. Dar, Michael S. Lopez, Kevan M. Shokat  Chemistry & Biology 
Crystallographic Structure of SurA, a Molecular Chaperone that Facilitates Folding of Outer Membrane Porins  Eduard Bitto, David B. McKay  Structure 
Crystal Structure of M. tuberculosis ABC Phosphate Transport Receptor
Volume 9, Issue 11, Pages (November 2001)
Volume 12, Issue 7, Pages (July 2004)
Volume 9, Issue 12, Pages (December 2002)
Volume 8, Issue 2, Pages (August 2001)
Volume 3, Issue 11, Pages (November 1995)
Volume 15, Issue 10, Pages (October 2008)
Volume 14, Issue 5, Pages (May 2007)
Glycerol Dehydrogenase
Volume 12, Issue 6, Pages (June 2004)
Volume 15, Issue 10, Pages (October 2008)
Volume 23, Issue 3, Pages (March 2016)
Volume 11, Issue 5, Pages (May 2003)
Crystal Structure of PMM/PGM
Crystal Structure of Human CD38 Extracellular Domain
Volume 15, Issue 10, Pages (October 2008)
Volume 16, Issue 10, Pages (October 2008)
Volume 133, Issue 1, Pages (April 2008)
The 1.9 Å Structure of α-N-Acetylgalactosaminidase
Structural Basis for the EBA-175 Erythrocyte Invasion Pathway of the Malaria Parasite Plasmodium falciparum  Niraj H. Tolia, Eric J. Enemark, B. Kim Lee.
Volume 90, Issue 1, Pages (July 1997)
Volume 15, Issue 9, Pages (September 2008)
Volume 17, Issue 6, Pages (June 2009)
Volume 33, Issue 2, Pages (January 2009)
Volume 2, Issue 8, Pages (August 1994)
Volume 19, Issue 11, Pages (November 2012)
Crystal Structure of Glycogen Synthase Kinase 3β
Volume 25, Issue 11, Pages e4 (November 2017)
Volume 20, Issue 11, Pages (November 2013)
Volume 12, Issue 7, Pages (July 2004)
Volume 6, Issue 3, Pages (March 1998)
Volume 21, Issue 1, Pages (January 2014)
Crystal Structure of Human CD38 Extracellular Domain
Aude Echalier, Celia F. Goodhew, Graham W. Pettigrew, Vilmos Fülöp 
Volume 91, Issue 7, Pages (December 1997)
Volume 25, Issue 8, Pages e3 (August 2017)
Volume 22, Issue 2, Pages (February 2014)
Activation Mechanism of the MAP Kinase ERK2 by Dual Phosphorylation
The Structural Basis for Catalysis and Specificity of the Pseudomonas cellulosa α- Glucuronidase, GlcA67A  Didier Nurizzo, Tibor Nagy, Harry J Gilbert,
The structure of the C-terminal domain of methionine synthase: presenting S- adenosylmethionine for reductive methylation of B12  Melinda M Dixon, Sha.
Structure and Mechanism of Imidazoleglycerol-Phosphate Dehydratase
Silvia Onesti, Andrew D Miller, Peter Brick  Structure 
Structure of the Rho Family GTP-Binding Protein Cdc42 in Complex with the Multifunctional Regulator RhoGDI  Gregory R. Hoffman, Nicolas Nassar, Richard.
Volume 20, Issue 9, Pages (September 2012)
Volume 13, Issue 10, Pages (October 2005)
Structural Insight into AMPK Regulation: ADP Comes into Play
Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with an Unusual Mode of Binding  David J. Sidote, Christopher.
Robert S. Magin, Glen P. Liszczak, Ronen Marmorstein  Structure 
Structural Basis of Swinholide A Binding to Actin
The Crystal Structure of an Unusual Processivity Factor, Herpes Simplex Virus UL42, Bound to the C Terminus of Its Cognate Polymerase  Harmon J Zuccola,
Arvin C. Dar, Michael S. Lopez, Kevan M. Shokat  Chemistry & Biology 
The Structure of JNK3 in Complex with Small Molecule Inhibitors
Volume 17, Issue 10, Pages (October 2009)
Structure of the EntB Multidomain Nonribosomal Peptide Synthetase and Functional Analysis of Its Interaction with the EntE Adenylation Domain  Eric J.
Volume 13, Issue 5, Pages (May 2005)
Luc Bousset, Hassan Belrhali, Joël Janin, Ronald Melki, Solange Morera 
Structure of the Histone Acetyltransferase Hat1
Volume 9, Issue 11, Pages (November 2001)
The Structure of T. aquaticus DNA Polymerase III Is Distinct from Eukaryotic Replicative DNA Polymerases  Scott Bailey, Richard A. Wing, Thomas A. Steitz 
Volume 16, Issue 7, Pages (July 2008)
Robert S. Magin, Glen P. Liszczak, Ronen Marmorstein  Structure 
Presentation transcript:

Volume 13, Issue 11, Pages 1143-1152 (November 2006) Insights into the Synthesis of Lipopolysaccharide and Antibiotics through the Structures of Two Retaining Glycosyltransferases from Family GT4  Carlos Martinez-Fleites, Mark Proctor, Shirley Roberts, David N. Bolam, Harry J. Gilbert, Gideon J. Davies  Chemistry & Biology  Volume 13, Issue 11, Pages 1143-1152 (November 2006) DOI: 10.1016/j.chembiol.2006.09.005 Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 1 The Catalytic Action of Two Family GT4 GTs (A) Schematic diagram of E. coli LPS. GlcN, D-glucosamine; Hep, L-glycero-D-manno-heptose; P, phosphate; EtNP, 2-aminoethyl phosphate; Glc, D-glucose; Gal, D-galactose. WaaG is responsible for the addition of the first glucose moiety via an α-1,3 glycosidic linkage to Hep II. (B) Structure of the antibiotic avilamycin A. Disruption of the avigt4 gene results in a product lacking the eurekanate moiety (boxed) normally bonded to the L-lyxose residue [8]. Chemistry & Biology 2006 13, 1143-1152DOI: (10.1016/j.chembiol.2006.09.005) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 2 Three-Dimensional Structures of Two Family GT4 GTs (A) Divergent (wall-eyed) stereo cartoon of the UDP-2FGlc complex of the GT4 enzyme WaaG from E. coli. The structure is “color-ramped” from N terminus (blue) to C terminus (red). (B) Divergent (wall-eyed) stereo cartoon of the UDP complex of the GT4 enzyme AviGT4 from Streptomyces viridochromogenes Tü57. This figure was drawn with MOLSCRIPT [42]. Chemistry & Biology 2006 13, 1143-1152DOI: (10.1016/j.chembiol.2006.09.005) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 3 Donor Site Interactions of E. coli GT4 WaaG (A) Observed electron density (calculated with the coefficients 2Fobs − Fcalc and αcalc) for the UDP-2F-glucose complex of E. coli WaaG (drawn with BOBSCRIPT [43]). (B) Schematic diagram of the interactions of WaaG with UDP-2F-glucose. Chemistry & Biology 2006 13, 1143-1152DOI: (10.1016/j.chembiol.2006.09.005) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 4 Overlay of the Active Centers of GT4 Enzymes AviGT4 and WaaG The UDP-2FGlc complex of WaaG is shown in cyan with AviGT4 in yellow. Electron density for the ordered UDP portion of the UDP-Glc in complex with AviGT4 is shown in red and is an electron density map calculated with the coefficients 2Fobs − Fcalc and αcalc and contoured at 1.2σ. AviGT4 residues discussed in the text are shown labeled. This figure was drawn with BOBSCRIPT [43]. Chemistry & Biology 2006 13, 1143-1152DOI: (10.1016/j.chembiol.2006.09.005) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 5 Electrostatic Surface Figures of WaaG and AviGT4 Surface representation of (A) WaaG and (B) AviGT4 colored by electrostatic potential (red, −3kT; blue +3kT, where k is the Boltzmann constant and T is temperature), calculated by the Adaptive Poisson-Boltzmann Solver (APBS) program [44] and visualized with Pymol (DeLano Scientific LLC, http://pymol.sourceforge.net/); ligands are shown in licorice representation. Chemistry & Biology 2006 13, 1143-1152DOI: (10.1016/j.chembiol.2006.09.005) Copyright © 2006 Elsevier Ltd Terms and Conditions

Figure 6 Overlay of GT-B Fold GTs (A) Phylogenetic tree displaying the distances among the 18 C-terminal domains of GTs and glycogen phosphorylases obtained from a multiple sequence alignment derived, in turn, from the structural superposition of the 3D coordinates. The phylogram was drawn with TreeView [41], and the structural superposition and alignment was carried out with SSM [39]. An asterisk indicates the unusual inverting sialyltransferase, which is found in the “retaining” side of the division (discussed in text). (B) Cartoon representation of the 3D alignment. Inverting GTs are colored red, while retaining GTs are colored blue. Two glucosyl recognition sites are highlighted in yellow: one derived from the retaining GT4 WaaG (this work), and the other in the inverting GT1 VvGT1 [14]. Chemistry & Biology 2006 13, 1143-1152DOI: (10.1016/j.chembiol.2006.09.005) Copyright © 2006 Elsevier Ltd Terms and Conditions