Proteomic analysis of externalized proteins

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Proteomic analysis of externalized proteins Proteomic analysis of externalized proteins. [35S]Methionine-labeled proteins synthesized by normal urothelium (cyst 473) or an invasive TCC (375-2 pT2-4 G4) and either maintained intracellularly (specimen) or released to the medium (released) were separated by 2D PAGE (IEF) and visualized by autoradiography. Proteomic analysis of externalized proteins. [35S]Methionine-labeled proteins synthesized by normal urothelium (cyst 473) or an invasive TCC (375-2 pT2-4 G4) and either maintained intracellularly (specimen) or released to the medium (released) were separated by 2D PAGE (IEF) and visualized by autoradiography. The area of the gel that contains the 14-3-3 β, σ, η, and ζ isoforms is shown enlarged with the positions for the respective isoforms indicated. José M. A. Moreira et al. Mol Cell Proteomics 2004;3:410-419 © 2004 The American Society for Biochemistry and Molecular Biology