Volume 117, Issue 3, Pages 595-604 (September 1999) Signal transduction–mediated adherence and entry of Helicobacter pylori into cultured cells  Bin Su, Staffan Johansson, Maria Fällman, Manuel Patarroyo, Marta Granström, Staffan Normark  Gastroenterology  Volume 117, Issue 3, Pages 595-604 (September 1999) DOI: 10.1016/S0016-5085(99)70452-X Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 1 Inhibition of H. pylori adherence to AGS cells by the tyrosine kinase inhibitor genistein. Cells were pretreated with genistein at different concentrations for 20 minutes before infection with H. pylori. Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 2 Time-dependent induction of tyrosine phosphorylation in AGS cells upon infection with H. pylori. Western blots from lysates of noninfected AGS cells or AGS cells infected with type I strain A5. The blots were probed with (A) antiphosphotyrosine antibody RC20H, (B) anti-p130Cas antibody, and (C) anti-FAK antibody. Note that AGS cells as some other cell lines can undergo autophosphorylation. Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 3 Effects of Yersinia YopH on H. pylori–induced tyrosine phosphorylation of the 125–130-kilodalton protein in AGS cells. Immunoblot (antiphosphotyrosine antibody, RC20H) from lysates of H. pylori only (lane 1); AGS cells infected with H. pylori A5 (lane 2); noninfected AGS cells (lane 3); AGS cells infected with Yersinia expressing active YopH (lane 4); AGS cells infected with Yersinia expressing inactive YopH (lane 5); AGS cells coinfected with Yersinia expressing inactive YopH and H. pylori A5 (lane 6); and AGS cells coinfected with Yersinia expressing active YopH and H. pylori A5 (lane 7). Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 4 Effects of Yersinia expressing YopH on type I H. pylori strain A5 adherence to AGS cells. AGS cells were preinfected with Yersinia strain MYMpyopH or its isogenic YopH mutant MYMpyopHC403A at a calculated bacteria/cell ratio of 20:1 or 100:1 at 37°C for 30 minutes and then infected with H. pylori A5 at a bacteria/cell ratio of 80:1 for an additional 30 minutes. Because of their different morphology, adherent H. pylori could be distinguished from adherent Yersinia. Only H. pylori was counted in the coinfections (right). Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 5 Effect of integrin expression, genistein, and integrin antibodies on H. pylori adherence. (A) Adherence of H. pylori strains A5 and P119 to β1-integrin–deficient and –transfected cell lines. ■, Not pretreated; ▨, 100 μmol/L genistein. (B) Effect of antibodies (concentration at 5 μg/mL) against different integrin subunits on the adherence of H. pylori A5 to AGS cells. Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 5 Effect of integrin expression, genistein, and integrin antibodies on H. pylori adherence. (A) Adherence of H. pylori strains A5 and P119 to β1-integrin–deficient and –transfected cell lines. ■, Not pretreated; ▨, 100 μmol/L genistein. (B) Effect of antibodies (concentration at 5 μg/mL) against different integrin subunits on the adherence of H. pylori A5 to AGS cells. Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 6 Effect of genistein (a tyrosine kinase inhibitor) and cytochalasin D (an inhibitor of actin polymerization) on the internalization of H. pylori into cell lines as well as into AGS cells. (A) β1-integrin–deficient and –transfected cell lines were pretreated with 100 μmol/L genistein and 2 μmol/L cytochalasin D before H. pylori infection. (B) AGS cells were pretreated with different concentrations of genistein and cytochalasin D before 2 type I strains and 1 type II H. pylori strain infection. Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions

Fig. 6 Effect of genistein (a tyrosine kinase inhibitor) and cytochalasin D (an inhibitor of actin polymerization) on the internalization of H. pylori into cell lines as well as into AGS cells. (A) β1-integrin–deficient and –transfected cell lines were pretreated with 100 μmol/L genistein and 2 μmol/L cytochalasin D before H. pylori infection. (B) AGS cells were pretreated with different concentrations of genistein and cytochalasin D before 2 type I strains and 1 type II H. pylori strain infection. Gastroenterology 1999 117, 595-604DOI: (10.1016/S0016-5085(99)70452-X) Copyright © 1999 American Gastroenterological Association Terms and Conditions