Retrospective molecular and phenotypic analysis of poliovirus vaccine strains isolated in Greece  V. Pliaka, M.E. Filliponi, Z. Kyriakopoulou, I.G.A. Ruether, D. Tsakogiannis, C. Gartzonika, S. Levidiotou-Stefanou, P. Markoulatos  Clinical Microbiology and Infection  Volume 17, Issue 10, Pages 1554-1562 (October 2011) DOI: 10.1111/j.1469-0691.2011.03470.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 One-step growth curve analysis of oral poliovirus vaccine isolates 522, 742 and MB1 in comparison with Sabin vaccine strains in Hep2 cells. Cells were infected at a multiplicity of infection of 10, and incubated at 37°C or 40°C. Total virus production at different times (0–16 h) post-infection was determined by TCID50 assay on Hep2 cells. Each point represents the mean (± standard deviation) of virus titres from five different experiments. Clinical Microbiology and Infection 2011 17, 1554-1562DOI: (10.1111/j.1469-0691.2011.03470.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions