Laser scanning analysis of the double-labeled immunofluorescence for 14-3-3σ (Alexa Fluor® 594; red) and p63 (Alexa Fluor® 488; green). Laser scanning.

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Laser scanning analysis of the double-labeled immunofluorescence for 14-3-3σ (Alexa Fluor® 594; red) and p63 (Alexa Fluor® 488; green). Laser scanning analysis of the double-labeled immunofluorescence for 14-3-3σ (Alexa Fluor® 594; red) and p63 (Alexa Fluor® 488; green). Original magnification, ×100 (A and D, SCC 536-1), ×800 (B and C, 916-1 pTa G1 and 817-5 pT2-4 G3, respectively), and ×630 (E and F, 916-1 pTa G1 and 817-5 pT2-4 G3, respectively); also ×630 for lower right panel inset in A (corresponding to the framed area in the lower left panel). This figure shows that 14-3-3σ expression in tumors does not correlate with the dominant-negative splice variant ΔNp63 (A–C) nor with the trans-activating variant TAp63α (D–F). José M. A. Moreira et al. Mol Cell Proteomics 2004;3:410-419 © 2004 The American Society for Biochemistry and Molecular Biology