Transcriptional Profiling of Psoriasis Using RNA-seq Reveals Previously Unidentified Differentially Expressed Genes Ali Jabbari, Mayte Suárez-Fariñas,

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Transcriptional Profiling of Psoriasis Using RNA-seq Reveals Previously Unidentified Differentially Expressed Genes Ali Jabbari, Mayte Suárez-Fariñas, Scott Dewell, James G. Krueger Journal of Investigative Dermatology Volume 132, Issue 1, Pages 246-249 (January 2012) DOI: 10.1038/jid.2011.267 Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Identification of a large set of differentially expressed genes in psoriatic skin. (a) Venn diagram comparing differentially expressed genes (DEGs) between lesional and non-lesional skin of psoriatic patients as assessed by RNA sequencing (RNA-seq) and microarray analysis using the samples from the same set of patients. Indicated in the diagram are the numbers of upregulated and downregulated DEGs. (b) Boxplots (means, boxes 25–75%, ± whiskers 95%) of the absolute values of the log2 fold change (FC) among 10 quantiles of concentrations for RNA-seq (left panel) and microarray analyses (right panel). (c) Scatterplot of the FC values for all genes as estimated by RNA-seq and microarray analyses. (d) Genes were grouped on the basis of dot plot location (left panel) and assessed for mean expression as determined by microarray analysis (right panel). Journal of Investigative Dermatology 2012 132, 246-249DOI: (10.1038/jid.2011.267) Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 RNA-seq identifies a novel set of DEGs in psoriasis skin lesions not otherwise identified in prior published studies. (a) Venn diagram comparing differentially expressed genes (DEGs) identified by RNA sequencing (RNA-seq) and those identified by published microarray studies. (b) Real-time RT-PCR confirmation of six upregulated (upper panel) and six downregulated (lower panel) DEGs identified by RNA-seq. Displayed are mean log2 fold change ±SEM, 15 paired lesional and control nonlesional samples for each gene. (c) SPRR2B protein expression was detected in psoriatic nonlesional (left) and lesional (right) skin sections by immunohistochemistry (× 100 magnification, three sets of paired samples assessed, bar=200μm). Journal of Investigative Dermatology 2012 132, 246-249DOI: (10.1038/jid.2011.267) Copyright © 2012 The Society for Investigative Dermatology, Inc Terms and Conditions