The vascular endothelial cell as a vehicle for gene therapy

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Presentation transcript:

The vascular endothelial cell as a vehicle for gene therapy Allan D. Callow, MD, PhD Journal of Vascular Surgery Volume 11, Issue 6, Pages 793-798 (June 1990) DOI: 10.1016/0741-5214(90)90075-L Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Robert R. Linton, 1975. Journal of Vascular Surgery 1990 11, 793-798DOI: (10.1016/0741-5214(90)90075-L) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Schematic representation of the cellular proliferative response after balloon denudation of the endothelium in the rat thoracic aorta. Endothelium is seen growing in from the ostia of the intercostal arteries diagrammatically depicted at the bottom of the diagram. Days 7 through 42 indicate days after injury. A and B represent degree of reendothelialization and the proliferative cellular response, respectively. Reendothelialization and fibromuscular thickening are interdependent. (Modified from Haudenschild and Schwartz. Lab Invest 1979;41:407-18.) Journal of Vascular Surgery 1990 11, 793-798DOI: (10.1016/0741-5214(90)90075-L) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Endothelial cells, stained and unstained, grown in tissue culture. Cells carrying the black-appearing pigment are actually stained blue from exposure of β-galactosidase to the substrate X-gal. Less than 50% of the cells have been successfully transduced. (From Callow AD. Impact of genetic engineering on vascular surgery. St. Louis: QMP (in press.). Journal of Vascular Surgery 1990 11, 793-798DOI: (10.1016/0741-5214(90)90075-L) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Canine endothelial cells transduced with the lacZ (β-galactosidase) marker gene and the second gene (neor) after exposure to G418. The surviving cells have been multiplied in tissue culture and carry a blue-staining cytoplasm reproduced here in black. Journal of Vascular Surgery 1990 11, 793-798DOI: (10.1016/0741-5214(90)90075-L) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Expression of t-PA by endothelial cells from the femoral and jugular veins and the carotid artery of the sheep transduced with a construct for human t-PA. No detectable amounts of t-PA were produced by the cells transduced with the BAG vector only. (From Dicheck DA, et al. Circulation 1989;80:1347-53.) Journal of Vascular Surgery 1990 11, 793-798DOI: (10.1016/0741-5214(90)90075-L) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions