Mapping rates of different transcript sets to the P

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Mapping rates of different transcript sets to the P Mapping rates of different transcript sets to the P. lambertiana genome (v1.0). Mapping rates of different transcript sets to the P. lambertiana genome (v1.0). Sequences were aligned to the P. lambertiana genome and the percentage of mapped transcripts was calculated at two combinations of coverage and sequence identity. (A) Transcripts obtained from the SMRT analysis for embryo (3-6 kb size-selected): transcripts identified as full-length (Pa), and set of transcripts after ICE/Quiver for isoform level clustering: consensus sequences (Pb1), low quality polished sequences (Pb2), and high quality polished sequences (Pb3); before (A1) and after (A2) transcript selection (CDS identification + clustering). (B) Pool embryo: all size selected (PacBio), HiSeq, and MiSeq (Illumina); before (B1) and after (B2) transcript selection. (C) Complete transcriptome set. Daniel Gonzalez-Ibeas et al. G3 2016;6:3787-3802 ©2016 by Genetics Society of America