Expression and Histone H4 Acetylation Levels of MAPK6 and WRKY53 in the HDT701 OX and RNAi Plants after M. oryzae Inoculation. Expression and Histone H4 Acetylation Levels of MAPK6 and WRKY53 in the HDT701 OX and RNAi Plants after M. oryzae Inoculation. NPB, Nipponbare.(A) and (B) Time-course transcription analysis of MAPK6 and WRKY53, respectively, after M. oryzae inoculation. Error bars indicate the sd from three biological replicates (n = 3), and asterisks indicate statistically significant differences compared with Nipponbare (NPB) (t test, *P < 0.05, **P < 0.01).(C) and (D) Time-course relative histone H4 acetylation level analysis on promoter region of MAPK6 and WRKY53, respectively, after M. oryzae inoculation. Diagrams of the MAPK6 and WRKY53 gene structures are shown above the ChIP analysis panel. Error bars indicate the sd from three biological replicates (n = 3), and asterisks indicate statistically significant differences compared with Nipponbare (t test, *P < 0.05, **P < 0.01).(E) Relative binding of HA-HDT701 to the promoters of UBQ, MAPK6, WRKY53, and WRKY71. Diagram of the Ubiquitin gene structure is shown above the ChIP analysis panel. The diagrams of the MAPK6, WRKY53, and WRKY71 gene structure are shown in (C), (D), and Supplemental Figure 6D online, respectively. Error bars indicate the sd from three biological replicates (n = 3), and asterisks indicate statistically significant differences compared with ubiquitin promoter region (UBQ-P) (t test, *P < 0.05, **P < 0.01). Bo Ding et al. Plant Cell 2012;24:3783-3794 ©2012 by American Society of Plant Biologists