Specific versus cooperative regulatory mechanisms of the cytokine genes that are clustered on the same chromosome Naoko Koyano-Nakagawa, PhD, Ken-ichi.

Slides:

Advertisements

Similar presentations

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Advertisements

Invest. Ophthalmol. Vis. Sci ;44(3): doi: /iovs Figure Legend:

Shin-Ichi Nishikawa, MD, PhD

A common intronic variant of CXCR3 is functionally associated with gene expression levels and the polymorphic immune cell responses to stimuli Jung-Won.

Suneet Agarwal, Anjana Rao Immunity

Suneet Agarwal, ScBa, João P.B. Viola, MD, PhDb, Anjana Rao, PhDa

An IgE-associated polymorphism in STAT6 alters NF-κB binding, STAT6 promoter activity, and mRNA expression Michaela Schedel, PhD, Remo Frei, PhD, Christian.

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

Roles of JAK kinases in human GM-CSF receptor signal transduction

The environmental pollutant pyrene induces the production of IL-4

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Christine M. Braun, BA, Shau-Ku Huang, PhD, Gregory G

Regulation of GM-CSF expression by the transcription factor c-Maf

Factors affecting the cytokine production of human T cells stimulated by different modes of activation Yoshio Harada, MDa, Sumiko Watanabe, PhDa, Hans.

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Differential influence of tyrosine residues of the common receptor β subunit on multiple signals induced by human GM-CSF Tohru Itoh, PhD, Rui Liu, MSc,

Blocking Sp1 Transcription Factor Broadly Inhibits Extracellular Matrix Gene Expression In Vitro and In Vivo: Implications for the Treatment of Tissue.

Estrogen enhances immunoglobulin production by human PBMCs

Functional characterization of the atopy-associated gene PHF11

Prostaglandin E2 control of T cell cytokine production is functionally related to the reduced lymphocyte proliferation in atopic dermatitis Sai Chan,

Granulocyte-macrophage colony-stimulating factor and IL-5 activate mitogen-activated protein kinase through Jak2 kinase and phosphatidylinositol 3-kinase.

Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles David Diaz-Sanchez, PhD, Albert Tsien, MD, Adrian.

Volume 11, Issue 6, Pages (December 1999)

IL-13 involvement in eosinophilic esophagitis: Transcriptome analysis and reversibility with glucocorticoids Carine Blanchard, PhD, Melissa K. Mingler,

High Mobility Group Protein I(Y) Is Required for Function and for c-Rel Binding to CD28 Response Elements within the GM-CSF and IL-2 Promoters S.Roy.

Human IL-31 is induced by IL-4 and promotes TH2-driven inflammation

HLX1 gene variants influence the development of childhood asthma

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Posttranscriptional regulation of IL-13 in T cells: Role of the RNA-binding protein HuR Vincenzo Casolaro, MD, PhD, Xi Fang, MD, Brian Tancowny, MS, Jinshui.

Analysis of antiapoptosis activity of human GM-CSF receptor

Eosinophil peroxidase stimulates the release of granulocyte-macrophage colony- stimulating factor from bronchial epithelial cells Shinji Motojima, MDa,

Glucocorticoids inhibit IL-4 and mitogen-induced IL-4Rα chain expression by different posttranscriptional mechanisms Lourdes Mozo, PhDa, Abel Gayo, BSa,

Role of Sp1 in Transcription of Human ATP2A2 Gene in Keratinocytes

Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T

β-Agonist enhances type 2 T-cell survival and accumulation

Gene structures of the α subunits of human IL-3 and granulocyte-macrophage colony- stimulating factor receptors: Comparison with the cytokine receptor.

The effects of glucocorticoids on human eosinophils

Keratinocyte growth factor promotes goblet cell differentiation through regulation of goblet cell silencer inhibitor Dai Iwakiri, Daniel K. Podolsky

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Molecular mechanisms of IgE regulation

Inflammatory cells, cytokine and chemokine expression in asthma immunocytochemistry and in situ hybridization Qutayba Hamid, MD, PhD, Editor Journal.

An Intronic Enhancer Driven by NF-κB Contributes to Transcriptional Regulation of Peptidylarginine Deiminase Type I Gene in Human Keratinocytes Shibo.

Manfred Fliegauf, PhD, Bodo Grimbacher, MD

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor Akihiko Muto, PhDa, Sumiko.

Epithelial cells as regulators of airway inflammation

Protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production Henk F. Kauffman, PhD, J.F.Chris.

Signals and nuclear factors that regulate the expression of interleukin-4 and interleukin- 5 genes in helper T cells Hyun Jun Lee, MSa, Ikuo Matsuda,

Characterization of a palindromic enhancer element in the promoters of IL4 , IL5 , and IL13 cytokine genes Sandra Codlin, PhD*, Cecilia Soh, PhD, Tak.

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

Volume 31, Issue 6, Pages (December 2009)

Ganglioside GQ1b enhances Ig production by human PBMCs

The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma James L. Lordan,

Human granulocyte-macrophage colony-stimulating factor (hGM-CSF)–dependent in vitro and in vivo proliferation and differentiation of all hematopoietic.

Regulation of human renin gene promoter activity: A new negative regulatory region determines the responsiveness to TNFα Ling-Sing K. Chen, Michael P.

MicroRNA-4443 regulates mast cell activation by T cell–derived microvesicles Irit Shefler, PhD, Pazit Salamon, PhD, Francesca Levi-Schaffer, PharmD, Adam.

Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms Toby J. Williams, Catherine A. Jones,

David M. Essayan, MD, Charity C

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Larry L. Thomas, PhD, Michelle D. Haskell, Emmanuel U

A thiol antioxidant regulates IgE isotype switching by inhibiting activation of nuclear factor-κB Yukiyoshi Yanagihara, PhD, Yuji Basaki, MSc, Keiichi.

Gp120- and TNF-α–induced modulation of human B cell function: Proliferation, cyclic AMP generation, Ig production, and B-cell receptor expression Christina.

Heterozygous N-terminal deletion of IκBα results in functional nuclear factor κB haploinsufficiency, ectodermal dysplasia, and immune deficiency Douglas.

Nuclear factor of activated T cells and YY1 combine to repress IL-5 expression in a human T-cell line Gretchen T.F. Schwenger, PhD, Régis Fournier, PhD,

Dexamethasone suppresses gene expression and production of IL-13 by human mast cell line and lung mast cells Toshiaki Fushimi, MD, Hiroshi Okayama, MD,

Transcriptional control of the IL-5 gene by human helper T cells: IL-5 synthesis is regulated independently from IL-2 or IL-4 synthesis Akio Mori, MD,

Differences in proliferation of the hematopoietic cell line TF-1 and cytokine production by peripheral blood leukocytes induced by 2 naturally occurring.

Transcriptional Regulation of the Elafin Gene in Human Keratinocytes

Keiji Miyazawa, MSc, Akio Mori, MD, PhD, Hirokazu Okudaira, MD, PhD

Update on glucocorticoid action and resistance

Volume 6, Issue 3, Pages (March 1997)

Presentation transcript:

Specific versus cooperative regulatory mechanisms of the cytokine genes that are clustered on the same chromosome Naoko Koyano-Nakagawa, PhD, Ken-ichi Arai, MD, PhD Journal of Allergy and Clinical Immunology Volume 98, Issue 6, Pages S174-S182 (December 1996) DOI: 10.1016/S0091-6749(96)70064-7 Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 1 Map of the cytokine gene cluster on human chromosome 5 (near segment q31) or the syntenic region on mouse chromosome 11. The relative locations of IL-13, IL-4, IL-5, IL-3, GM-CSF, and IL-9 genes are shown. Distances are not accurate and the orientation of each gene is unknown (except that IL-3 and GM-CSF are 10 kilobases apart, IL-13 and IL-4 are 12 kilobases apart, and IL-3 and GM-CSF, and IL-4 and IL-13 are transcribed in the same orientation, respectively). The human IL-4 and IL-5 genes are 110 to 180 kilobases apart from one another and are at least 600 kilobases from the IL-3 and GM-CSF genes.25 Journal of Allergy and Clinical Immunology 1996 98, S174-S182DOI: (10.1016/S0091-6749(96)70064-7) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 2 cis-acting DNA elements required for cytokine gene expression. Sequences that bind NF-AT family proteins are shown in black boxes. Journal of Allergy and Clinical Immunology 1996 98, S174-S182DOI: (10.1016/S0091-6749(96)70064-7) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 3 Physical map of the mouse IL-13/IL-4 gene region. A 40 kilobase-cosmid clone #129 containing mouse IL-13 and IL-4 genes was analyzed using restriction mapping. The map was verified by Southern hybridization of the genomic DNA. Journal of Allergy and Clinical Immunology 1996 98, S174-S182DOI: (10.1016/S0091-6749(96)70064-7) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 4 Transcription activity of plasmids carrying various chimeric promoter units. The GM-κB/GC-box and CLE0 are sequences from the GM-CSF promoter. The CT/GC-rich region is from the IL-3 promoter. The NF-κB sequence is from the immunoglobulin enhancer. Each chimeric promoter linked to the chloramphenicol acetyltransferase (CAT) gene was transfected to Jurkat cells by the DEAE-dextran method.26 Cells were stimulated with 20 ng/ml of PMA and 0.5 mM of A23187 40 hours after transfection, and cultured for an additional 8 hours. Transcriptional activity was determined by measuring CAT activity; bars represent unstimulated (solid), A23187/PMA-treated (hatched), and Tax-cotransfected (stippled) cells, respectively. Transfections were repeated at least three times. Each of the CAT activities was normalized to that of GM-95 stimulated with PMA/A23187, and the average is shown. Journal of Allergy and Clinical Immunology 1996 98, S174-S182DOI: (10.1016/S0091-6749(96)70064-7) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 5 Effect of EGR1, EGR2, and DB1 on the IL-3 promoter activity. Plasmids containing the 5’ upstream region of the IL-3 gene, IL-3 NheL (from position -650), del 14 (position -86), del 15 (position -56) were cotransfected to Jurkat cells with vector alone (-), or with the expression plasmid for DB1, EGR1, or EGR2. Cells were stimulated at 40 hours, and harvested at 48 hours after transfection. IL-3 NheL and del 14 but not del 15 contain the CT/GC-rich region. Journal of Allergy and Clinical Immunology 1996 98, S174-S182DOI: (10.1016/S0091-6749(96)70064-7) Copyright © 1996 Mosby, Inc. Terms and Conditions

FIG. 6 Cotransfection effect of Tax with EGR1, EGR2, and DB1 on the IL-3 promoter. Tax and the expression vector of EGR1, EGR2, or DB1 were cotransfected to Jurkat cells with IL-3 reporter plasmids. Cells were stimulated at 40 hours and harvested at 48 hours after transfection. Journal of Allergy and Clinical Immunology 1996 98, S174-S182DOI: (10.1016/S0091-6749(96)70064-7) Copyright © 1996 Mosby, Inc. Terms and Conditions