4/16 Daily Catalyst Pg. 53 DNA Extraction Lab 1. Label the parts of DNA: 2. Provide an example of a deletion mutation. 3. In gel electrophoresis, DNA separates based on _________. 4. What gives DNA is charge? A B C
4/16 Daily Catalyst Pg. 53 DNA Extraction Lab Quiz #12 DNA on Friday DNA test 4/22 Study guide due test Review day on 4/21 DNA Extraction lab on Thursday Be here! Labs are test grades! Interims come out this Friday Signed for extra credit Parent Night on Thursday 6-7:30 Extra Credit
4/16 Agenda Pg. 53 DNA Extraction Lab Daily Catalyst Class Business Objective DNA extraction lab Clean up Homework: lab report
DNA Recap 1. What are the three parts to DNA? Sugar, phosphate, and base 2. In base pairing, A pairs with _______ and C pairs with ____. A-T C-G 3. Who discovered DNA? Watson, Crick, and Franklin 4. What is the structure of DNA? Double helix
4/16 Objective Today, we will demonstrate the presence of DNA in eukaryotic cells using a DNA extraction technique.
Key Point #1: Extraction: To remove
Key Point #2: Living Cells Prokaryotes Eukaryotes Bacteria Contain DNA Double or single helix Animals and Plants Contain DNA Double helix
DNA Extraction Key Point #3: Key Point #4: DNA is extracted from the nucleus Key Point #4: The nuclear membrane must be broken to release the DNA Break the phospholipid membrane
Rules for the lab We will stay with our assigned group at our assigned lab table. We will read ALL directions before beginning the lab All materials will be used safely and properly Materials will be returned to their proper place and CLEANED Our lab space will be spotless at the end of the lab Failure to violate one of the rules will be immediate removal from the classroom and a score of a zero on the lab
Lab Groups Lab 1 Rilda & Kaitlyn Romel & Malika Lab 2 Diana & Victoria Strawberry Banana Lab 1 Rilda & Kaitlyn Romel & Malika Lab 2 Diana & Victoria Kyla & Mario Lab 3 Christian & Hannah O. Odalys & Mohammad Lab 4 Lawren & Mr. Kaki Irma & Julianna Lab 5 Sierra & Tiesha Michelle & Camila Esther & Lavonne Hannah L., James & Joseph
DNA extraction lab Today, we will extract DNA from fruit. Each group will have a fruit piece then we will compare results at the end. For this lab to be successful, all directions need to be carefully read.
DNA extraction lab Directions: With your partner, complete the pre-reading and pre-lab questions. Assign the jobs to your partnership and create the perfect hypothesis for an amazing lab report. Time: 10 minutes Noise: 1 (whispers)
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