Messenger RNA expression for the follicle-stimulating hormone receptor and luteinizing hormone receptor in human oocytes and preimplantation-stage embryos

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Presentation transcript:

Messenger RNA expression for the follicle-stimulating hormone receptor and luteinizing hormone receptor in human oocytes and preimplantation-stage embryos Eleni Patsoula, PhD, Dimitris Loutradis, MD, Peter Drakakis, MD, Lina Michalas, MD, Ritsa Bletsa, RN, Stylianos Michalas, MD Fertility and Sterility Volume 79, Issue 5, Pages 1187-1193 (May 2003) DOI: 10.1016/S0015-0282(03)00071-2

FIGURE 1 FSHR mRNA expression in human oocytes and preimplantation embryos. M: 100 bp DNA marker; lane 1: unfertilized oocytes with and without RT, respectively (±); lane 2: zygotes (±); lane 3: 2-cell embryos (±); lane 4: 4-cell embryos (±); lane 5: morula-stage embryos (±); lane 6: blastocyst-stage embryos (±). Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003. Fertility and Sterility 2003 79, 1187-1193DOI: (10.1016/S0015-0282(03)00071-2)

FIGURE 2 RT-PCR amplification of LHR mRNA in human oocytes and preimplantation embryos. M: 100 bp DNA marker; lane 1: unfertilized oocytes with and without RT, respectively (±); lane 2: zygotes (±); lane 3: 2-cell embryos (±); lane 4: 4-cell embryos (±); lane 5: morula-stage embryos (±); lane 6: blastocyst-stage embryos (±). Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003. Fertility and Sterility 2003 79, 1187-1193DOI: (10.1016/S0015-0282(03)00071-2)

FIGURE 3 (A) Gel electrophoresis of RT-PCR products for FSHR with mRNA from cumulus cells and with mRNA from culture medium. FSHR RT-PCR analysis with mRNA from cumulus cells surrounding oocytes (lane 1) and with mRNA from cumulus cells surrounding zygotes (lane 2). RT-PCR analysis with culture medium of 2-cell embryos (lane 3), 4-cell embryos (lane 4), morulae (lane 5), and blastocysts (lane 6). (B) Gel electrophoresis of RT-PCR products for LHR with mRNA from cumulus cells and with mRNA from culture medium. LHR RT-PCR analysis with mRNA from cumulus cells surrounding oocytes (lane 7) and with mRNA from cumulus cells surrounding zygotes (lane 8). RT-PCR analysis with culture medium of 2-cell embryos (lane 9), 4-cell embryos (lane 10), morulae (lane 11), and blastocysts (lane 12). A 100-bp molecular size marker was used. Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003. Fertility and Sterility 2003 79, 1187-1193DOI: (10.1016/S0015-0282(03)00071-2)

FIGURE 4 Restriction enzyme digest of the second-round 340-bp FSHR PCR product (A) and the 191-bp LHR PCR product (B) with the Hind III enzyme. Restriction products are indicated with arrows. A 100-bp molecular size marker was used. Patsoula. FSH, LH receptors in human pre-embryos. Fertil Steril 2003. Fertility and Sterility 2003 79, 1187-1193DOI: (10.1016/S0015-0282(03)00071-2)